Abstract During the feeding of mulberry silkworms, when temperature in larvae fluctuates sharply and amount nutrients fed is below or above norm, metabolism physiological processes body are disrupted. As a result, silk cocoon water balance, respiration, digestion absorption impaired, activity glands creating conditions for disease-causing microorganisms. In this case, growth silkworms by 4 time...

Four new okaramine congeners, okaramines J, K, L and M, were isolated from okara fermented with Penicillium simplicissimum ATCC 90288, together with five biogenetically related compounds, cyclo (8a-(α,α-dimethylallyl)-L-Trp-8a-(α,α-dimethylallyl)-L-Trp), cyclo (N (8)-(α,α-dimethylallyl)-L-Trp-6a'-(α,α-dimethylallyl)-L-Trp), cyclo (L-Trp-L-Trp), cyclo (6a'-(α,α-dimethylallyl)-L-Trp-L-Trp), and c...

The minichromosome maintenance protein (MCM) family is involved in the regulatory role of DNA replication in eukaryotic organisms. A cDNA encoding of an MCM of the silkworm, Bombyx mori L. (Lepidoptera: Bombycidae), was cloned by reverse transcriptase-polymerase chain reaction (RT-PCR) and sequenced. The resultant amino acid sequence and phylogenetic analysis revealed high identity to MCM, and ...

The silkworm, Bombyx mori is infected by a number of viruses: Bombyx mori nuclear polyhedrosis virus (BmNPV), cytoplasmic polyhedrosis virus (CPV), infectious flacherie virus (IFV) and densonucleosis virus (DNV). Except DNV, resistance of silkworms to the virus is controlled by polygenes. Serologically, two DNV types, DNV-1 and DNV-2 are identified. The non-susceptibility to DNV types is confer...

To explore efficient of different gene transfer methods and to express human granulocyte-macrophage colony-stimulating factor (hGM-CSF) in silk glands of transgenic silkworm, a novel transgenic vector containing a hGM-CSF controlled by silkworm fibroin heavy chain gene promoter (Pfib-H) with a neomycin resistance gene (neo) driven by BmNPV (B. mori nucleopolyhedrovirus) immediate-early gene ie-...

The utility of inter simple sequence repeat-PCR (ISSR-PCR) assay in the genetic characterisation and elucidation of the phylogenetic relationship of different microsporidian isolates infecting tropical tasar silkworm, Antheraea mylitta Drury, is demonstrated. A total of 22 different microsporidians collected from the diseased tasar silkworms from Jharkhand state of India were analysed using mor...

The tropical tasar silkworm, Antheraea mylitta, is a semi-domesticated vanya silk-producing insect of high economic importance. To date, no molecular marker associated with cocoon and shell weights has been identified in this species. In this report, we identified a randomly amplified polymorphic DNA (RAPD) marker and examined its inheritance, and also developed a stable diagnostic sequence-cha...

Human serum albumin (HSA) is an important biological preparation with a variety of biological functions in clinical applications. In this study, the mRNA of a fusion transposase derived from the pESNT-PBase plasmid and a pBHSA plasmid containing the HSA gene under the control of a fibroin light chain (FL) promoter were co-injected into fertilized eggs. Fifty-six transgenic silkworm pedigrees ex...

In animals, oral administration of the cholera toxin B (CTB) subunit conjugated to the autoantigen insulin enhances the specific immune-unresponsive state. This is called oral tolerance and is capable of suppressing autoimmune type 1 diabetes (T1D). However, the process by which the CTB-insulin (CTB-INS) protein works as a therapy for T1D in vivo remains unclear. Here, we successfully expressed...