In order to determine the involvement of nitric oxide in the pathogenesis of coccidiosis induced by Eimeria, 30 chickens were challenged with mixed sporulated oocysts of four species of Eimeria (E. acervulina, E. maxima, E. necatrix, and E. tenella) at 26 days of age. There was an increasing of oocyst shedding in the infected birds at 6, 10 and 14 days post-infection. Histopathological examinat...

The relationship between acetaminophen (APAP) reactive metabolite formation, nitrotyrosine (NT) production, and cytokine elevation in APAP toxicity was investigated. Mice were dosed with 300 mg/kg of APAP and sacrificed at 1, 2, 4, 8, and 12 h. Serum aspartate aminotransferase (AST) was elevated by 4 h. The relative amount of NT correlated with toxicity and was localized in the necrotic cells. ...

1. Filtrates from B. coli, B. typhosus, or meningococci injected into the skin of guinea pigs do not produce visible inflammation. When these injections are followed by intravascular injections of the same material, hemorrhages do not occur in the skin. 2. Guinea pigs sensitized to horse serum react with redness and edema to 0.1 or 0.01 cc. of horse serum injected into the skin, and subsequent ...

Leptin administration has been shown to increase renal, adrenal, and lumbar sympathetic nerve activity. However, this generalized sympathoexcitatory activity is not always followed by an increase in arterial pressure. The present study tested the hypothesis that leptin induces a release of nitric oxide (NO) that opposes the pressor effect of sympathoexcitation. The effect of intravenous adminis...

The effects of probiotic bacteria Enterococcus faecium (EF) and selenium were studied on methotrexate (MTX) treatment in rats with adjuvant arthritis (AA). Arthritic rats were preventive treated orally with the following substances: lyophilized EF (15mg/kg/day, 5 days a week); sodium selenite pentahydrate (SSe, 0.050mg/kg containing 0.015 mg/kg selenium, 5 days a week); MTX (0.6 mg/kg/week), an...

BACKGROUND Stable plasma nitric oxide (NO) metabolites (NOM), composed predominantly of nitrate and nitrite, are attractive biomarkers of NO bioavailability. NOM levels integrate the influence of NO-synthase-derived NO production/metabolism, dietary intake of inorganic nitrate/nitrite, and clearance of NOM. Furthermore, nitrate and nitrite, the most abundant NOM, can be reduced to NO via the ni...

Recent animal data suggest that reduced lipoic acid (LA) prevents oxidative inhibition of the nitrate bioactivating enzyme, the mitochondrial aldehyde dehydrogenase (ALDH-2), and that pentaerythritol tetranitrate (PETN) does not induce nitrate tolerance because of its intrinsic antioxidative properties, thereby preserving ALDH-2 activity. We sought to determine whether ALDH-2 activity in circul...

BACKGROUND Early childhood introduction of nutritional habits aimed at atherosclerosis prevention reduces children's serum total cholesterol concentration, but its effect on vascular endothelial function is unknown. METHODS AND RESULTS Between 1990 and 1992, we randomized healthy 7-month-old infants (n=1062) to intervention (low-saturated-fat diet) and control (unrestricted diet) groups. At t...

Exercise, alone or combined with changes in lifestyle, can prevent or reduce the need for pharmacotherapy in patients with compromised endothelium-dependent function. The aim of this study was to examine the protective effect of aerobic exercise against (L-NAME)-kidney damage in male rats induced by Nω-nitro-L-arginine methyl ester (L-NAME). L-NAME was administered to rats intraperitoneally in ...

Development of severe sepsis is thought to result from the overproduction of cytokines, such as tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta (IL-1beta), and nitric oxide. Recently, 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitors, which are antihypercholesterolemic agents, have been reported to inhibit lipopolysaccharide (LPS)-induced production of cytokines and nitri...