Measurement of immunoglobulins (Ig) and of complement component C3 in human serum by automated and manual nephelometric techniques is tedious, and the effective linear range is too narrow. We describe a laser nephelometer/reagent system for measuring serum proteins by the use of forward light scatter (which enhances the ratio of reaction/blank), electronic blank subtraction, laser light (632 nm...

Manual procedures are presented for determining immunoglobulins IgG, IgA, and 1gM in human serum. The three immunoglobulins are quantitated, after reaction with specific ant sera, by measuring light scattering by the antigen-antibody complexes. The procedure is standardized by use of pooled human serum with known concentrations of IgG, IgA, and 1gM. Results with the present system correlated ex...

The technique of immunoelectrophoresis (IEP) is widely employed in the qualitative analysis of serum immunoglobulins. The most commonly used support media are agarose or agar gels, but the mobility of immunoglobulins is different in these two media. The presence of a small amount of a cathodal monoclonal immunoglobulin G may not be detected on IEP in agar if it is masked by larger amounts of po...

bronchiectasis is a chronic debilitating condition characterized by abnormal dilated thick-walled bronchi. to investigate humoral immune function in bronchiectatic patients, this study was performed . forty patients with established diagnosis of bronchiectasis, who were referred from two tertiary care pulmonology centers in tehran, were investigated in this study. immunoglobulin isotypes concen...

BACKGROUND Thalassemia is the most common anemia with hereditary base in Iran. The aim of this study was to evaluate the humoral immune system and assess the effect of splenectomy on the serum level of immunoglobulins IgG, IgM, and IgG and complement components in patients with major thalassemia. METHODS This quasi-experimental study (before-after) was performed on 40 patients with major thal...

Both anesthesia and major surgeries may affect the immune system, especially the cell-mediated immune responses, but also the humoral immunity. Our goal was to assess the effect of propofol and isoflurane on the serum concentration of immunoglobulins G, M, A and E in patients undergoing off-pump coronary artery bypass graft (CABG) surgery. This study was a double-blinded randomized clinical tri...

We report here the case of a 73-year-old woman with chronic lymphocytic leukemia. Two years after the diagnosis, electrophoresis of her serum showed two monoclonal fractions, but our modified immunofixation procedure revealed four monoclonal immunoglobulins in five fractions: two IgG lambda fractions, one IgG kappa fraction, one IgA kappa fraction, and one IgG kappa fraction. This is an excepti...