Post-natal testicular development is dependent on gonadotrophin and androgen stimulation. Follicle stimulating hormone (FSH) acts through receptors (FSHR) on the Sertoli cell to stimulate spermatogenesis while androgens promote testis growth through receptors (AR) on the Sertoli cells, Leydig cells and peritubular myoid cells. In this study we have examined the effects on testis development of ...

Sertoli cell tumors of the testis are extremely rare tumors with a heterogeneous pathology. Three histological variants have been described: Sertoli cell tumor not otherwise specified (NOS), large cell calcifying sertoli cell tumor and the Sclerosing Sertoli cell tumor. The sclerosing Sertoli cell tumor described herein is associated with prominent stromal sclerosis. They present as painless...

Experimental hypothyroidism during sexual development Testes size and number of spermatozoa in adulthood are determined by postnatal/pubertal proliferation of seminal tubule somatic cell constituents, the Sertoli cells. Cessation of proliferation of Sertoli cells during early development occurs concomitantly with formation of their terminally differentiated population. Transient experimental hy...

AIM To determine the effectiveness of the sk11, sk9 and sk11 TNUA5 Sertoli cell lines in binding germ cells in vitro. METHODS The immortalized Sertoli cell lines sk9, sk11 and sk11 TNUA5 were used in co-culture experiments with germ cells in media with or without reproductive hormones and incubated for 44 h at 32 degrees . The number of germ cells bound to Sertoli cells was then determined an...

The Sertoli cell intermediate filament cytoskeleton is composed of the type III family member vimentin. The distribution of Sertoli cell vimentin varies with the stage of spermatogenesis, with shortening of the filaments at stages VII-VIII, the stages of spermiation. Experimental reduction in intratesticular testosterone (T) concentration also results in the sloughing of advanced spermatids fro...

introduction: morphology and ultra-structure of myoid and sertoli cells were studied in patients with non-obstructive azoospermia who were admitted to testicular sperm extraction (tese) and intracytoplasmic sperm injection (icsi), by transmission electron microscopy (tem). materials and methods: 12 biopsies of testicular tissues from patients with non-obstructive azoospermia referred to royan i...

The testicular vasculature forms a complex network, providing oxygenation, micronutrients, and waste clearance from the testis. The vasculature is also instrumental to testis function because it is both the route by which gonadotropins are delivered to the testis and by which T is transported away to target organs. Whether Sertoli cells play a role in regulating the testicular vasculature in po...

Spermatogonial stem cells (SSCs) maintain spermatogenesis throughout life in the male. Maintenance of SSCs and induction of spermiogenesis in vitro may provide a therapeutic strategy to treat male infertility. This study investigated in vitro differentiation of mouse SSCs in presence or absence of Sertoli cells, hormones and vitamins. Spermatogonial populations were enriched from testes of 4-6 ...

Proliferation and cord formation by embryonic Sertoli cells are pivotal events involved in testis morphogenesis. A number of growth factors have been implicated in mediating these events. However, the exact level of involvement and importance of each as yet remains elusive. We have adopted an in vitro approach to assess developing mouse Sertoli cells, whereby they are cultured in the presence o...

Although many genes are expressed selectively in Sertoli cells, regulatory sequences sufficient to drive Sertoli cell-specific expression in the postnatal and adult testis in vivo have not been identified. In the present study, we identified promoter sequences from the Pem homeobox gene that direct Sertoli cell-specific expression in an androgen-dependent and stage-specific manner. Immunohistoc...