Objective: To measure the healthcare utilization, induced labour and frequency of C-sections in pregnancy after stillbirth tertiary care setting.
 Study Design: Comparative prospective study.
 Place Duration Study: Pak Emirates Military Hospital, Rawalpindi Pakistan, from Sep 2018-Sep 2019.Methodology: A total 181 pregnant women were recruited divided into two groups; Group-1=75pregna...

Two methods of purification of the plantaricin ST31, a bacteriocin produced by Lactobacillus plantarum ST31 are used in this study – the method of ammonium sulfate precipitation, Sep-pack C18 cartridge and reverse-phase HPLC chromatography on C18 Nucleosil column, and the method of direct purification by cation exchange SP Sepharose Fast Flow column Amersham (Pharmacia Biotech). The purity of t...

Mammalian cell proteins, modified post-translationally by derivatives of [3H]mevalonic acid, were subjected to methylation and sulfonium salt cleavage reactions previously used to release isoprenoids from cysteine residues in yeast peptides. The labeled isoprenoid extracted into chloroform comigrated with farnesol through a series of chromatography steps including Sep-Pak C-18 fractionation, si...

The levels of free pyrroloquinoline quinone (PQQ) in various foods were examined by the use of gas chromatography-mass spectrometry. PQQ was extracted from the samples, after addition of [U-13C]PQQ as internal standard, with n-butanol and Sep-Pak C18 cartridges. After derivatization of PQQ with phenyltrimethylammonium hydroxide, molecular peaks at m/z 448 and 462 were used for detection of PQQ ...

A novel porous organosilicate material was evaluated for application as a solid phase extraction sorbent for preconcentration of nitroenergetic targets from aqueous solution prior to HPLC analysis. The performance of the sorbent in spiked deionized water, groundwater, and surface water was evaluated. Targets considered included 2,4,6-trinitrotoluene, 2,4-dinitrotoluene, RDX, HMX, and nitroglyce...

A method was developed for the fractionation of grape and wine proanthocyanidins according to their degree of polymerization. The grape and wine proanthocyanidins were fractionated on C18 Sep-Pak cartridges into three fractions by different organic solvents. The combination of TLC, analytic HPLC, and degradation with toluene-R-thiol confirmed that these three fractions contained, respectively, ...

A competitive, double antibody enzyme immunoassay for oxytocin in a heterologous system was developed. Horseradish peroxidase was conjugated with oxytocin using N-succinimidyl 3-(2-pyridyldithio) propionate, and rabbit anti-oxytocin serum was produced by immunization of oxytocin-bovine serum albumin complex which was prepared by the carbodiimide method. The sensitivity of the assay was 4 microI...