At room temperature, the fluorescence of chloroplasts in vivo and in vitro originates nearly exclusively from the chlorophyll of the oxygen evolving system II of the photosynthetic apparatus. The yield and the kinetics of this light emission are closely coupled to the primary photochemical events of photosystem II. Fluorescence studies, therefore, have contributed much to our knowledge about th...

Covalent fluorescent probes were used to label the rabbit intestinal brush border Na+-glucose cotransporter at the putative glucose and Na+ binding sites, and a steady-state fluorescence energy transfer technique was used to measure the distance between the two binding sites. In both intact brush border membrane vesicles and partially purified soluble protein, the distance (R2/3) between the Na...

objective(s): there is controversial evidence about the effect of methamphetamine (meth) on spatial memory. we tested the time- dependent effects of meth on spatial short-term (working) and long-term (reference) memory in meth –sensitized and withdrawn rats in the morris water maze. materials and methods: rats were sensitized to meth (2 mg/kg, daily/5 days, sc). rats were trained in water maze ...

Objectives: In previous studies the therapeutic effects of Nigella sativa have been demonstrated on asthmatic animals. In the present study, the preventive effect of single dose of alpha-hederin, its active constituent, has been evaluated on lung inflammation and some inflammatory mediators in lungs of ovalbumin sensitized rat in order to elicit its mechanism. Materials and methods: Forty rats ...

Sensing ion or ligand concentrations, physico-chemical conditions, and molecular dimerization or conformation change is possible by assays involving fluorescent lifetime imaging. The inherent low throughput of imaging impedes rigorous statistical data analysis on large cell numbers. We address this limitation by developing a fluorescence lifetime-measuring flow cytometer for fast fluorescence l...

Excitons in ideal aromatic crystals are delocalized. So far no experimental evidence for self trapping of excitons is known. Trapping of energy is possible in mixed crystals or in crystals containing defects. Disturbed exciton states due to the presence of foreign molecules or due to structural defects (the so-called X-traps) are the most characteristic type of defects in aromatic crystals. Som...

To date, thermally activated delayed fluorescence-sensitized fluorescent organic light-emitting diodes (TSF-OLEDs) have undergone substantial research to achieve high efficiency and good operational stability in wide color gamut regions. Usually, a highly efficient TSF device, the Förster resonance energy transfer rate (kFRET) should be enhanced, whereas Dexter (kDET) suppressed. Even though de...

Methods Wild-type and Sema4C mice were sensitized intraperitoneally using 100 μL OVA (0.5 mg/mL ovalbumin and 4 mg/mL aluminum hydroxide in PBS) on days 0 and 14, and were challenged intranasally using 20 μL OVA (10 mg/mL ovalbumin in PBS) from days 28 to 30. Sacrifice and analysis of Airway Hyperresponsiveness via flexiVent was performed on day 31. Serum IgE and IL-10 expression levels were me...

background the imbalance in the t helper (th) 1/th2 and th1/t regulatory (treg) cells-related immune responses plays a key role in pathogenesis of allergic asthma. objectives the aim of this study was to evaluate the effects of cpg-oligodeoxynucleotide (cpg-odn), monophosphoryl lipid a (mpla) and bacillus calmette-guerin (bcg) on the ratio of th1/th2 and th1/treg cells-related parameters in an ...