Storage of buffalo (Bubalus bubalis) bull semen in the cryopreserved state is discussed in this article. Fertility rate in buffalo following artificial insemination with frozen-thawed semen is reviewed. To better understand the freezability of bubaline spermatozoa, the available data on biochemical components and the activity of specific enzymes of semen/spermatozoa are given. Moreover, the maj...

s / Journal of Equine Veterinary Science 34 (2014) 76 76 x106sperm/mL. Sperm recovery rate after centrifugation was lower in low concentration samples (p<0.001). Mean lactate concentration at T0 was the same in all three groups and directly correlated with raw semen volume (r1⁄40.5032; p1⁄40.0103). Lactate concentrations at T0 and T24 were not correlated. Compared to T0, lactate concentration w...

The objective of this study was to compare the effect of Butylated Hydroxy Toluene (BHT), Pentoxifylline (PTX) and α-tocopherol (Vit E) on semen quality parameters of Karan Fries bulls. The fortification of extender by various semen additives improves motility as well as fertility of spermatozoa. Split samples of 24 ejaculates of four Karan Fries bulls were extended in extender with or without ...

The goal of this study was to examine the effect of caffeine on motility parameters of cooling-stored ram sperm – total motility and progressive movement. Caffeine is a cyclic nucleotide phosphodiesterase inhibitor, which can affect sperm motility. The semen was kept at 4 ̊C for several days. Caffeine was added to fresh ram semen diluted in Triladyl at concentrations of 1, 2 and 4 mmol.l -1 ever...

A field trial was conducted on several hundred farms in The Netherlands to compare the fertilizing capacity of boar spermatozoa stored for 1, 2, or 3 d at 18 C in either Kiev or Beltsville liquid extender (BL-1). Two storage volumes (25 and 100 ml) were used. Twelve Dutch Landrace boars, all in routine commercial artificial insemination service, provided the semen in twice-a-week collections. O...

Basic concepts of cryopreservation and the causes of cryoinjury are reviewed. The possible roles of cryoprotectants and additives are considered in the context of their putative interactions with the sperm plasma membrane. Modern approaches to the laboratory assessment of spermatozoa after freeze-thawing are also briefly discussed.

the purpose of this study was to determine the effects of different concentrations of vitamin e (vit. e) in the quality of preserved stallion semen at 5ᵒc for a period of 48 h. semen was collected and most (≥90%) seminal plasma removed by centrifugation at 600 × g. pooled samples divided in four equal parts and diluted with extenders that was supplemented with different concentrations of vit. e...

antioxidant effect of olive leaf extract (ole) was studied on motility, viability, plasma membrane integrity of spermatozoa and malondialdehyde production in 12 ross 308 roosters at their 30 weeks of age. semen samples were collected by abdominal massage in 5 times. in each session after the initial sperm assessment, collected samples were pooled and diluted with sexton extender.samples were sp...

The purpose of this study was to evaluate the probable effects of the vitamin E addition in different levels to the extender of chicken semen on spermatozoa quality during storage of semen at 4°C for 0, 3, 6, 10 and 24 hours. Eight young Ross broiler breeder strain 308 roosters were used in this experiment. The collected semen from all roosters was mixed together and diluted with modified a Rin...