BACKGROUND The compaction of human sperm chromatin is the result of replacement of approximately 85% of histones with protamines. Germ-line testis/sperm-specific histone 2B (TSH2B) has been detected in only approximately 30% of mature spermatozoa. Its level in the semen of subfertile patients varies; its function is unknown. We evaluated TSH2B in the sperm samples of 23 donors and 49 subfertile...

A total of 28 donor semen samples were used to evaluate the characteristics of laboratory variability in measuring reactive oxygen species (ROS). The objectives of this study were to assess the interassay (same sample observed on different days by the same observers) variability; interdonor, intraobserver (replications of the same sample on the same day) variability; and interobserver (multiple...

Transplantation of male germ line cells into sterilized recipients has been used in mammals for conventional breeding as well as for transgenesis. We have previously adapted this approach for the domestic chicken and we present now an improvement of the germ cell transplantation technique by using an enriched subpopulation of c-Kit-positive spermatogonia as donor cells. Dispersed c-Kit positive...

Received: Revised: Accepted: April 22, 2013 July 30, 2013 July 31, 2013 The present study was aimed to investigate the association of scrotal circumference (SC) with sperm production and semen quality in Sahiwal bulls. Mature semen donor bulls (n=76), 3 to 5 years old, were divided into three groups according to SC: GI, GII, and GIII with 28-30, 31-35, and >35 cm, respectively. Semen was collec...

Introduction To study whether paternal age exerts an effect, independent of maternal age, on the outcomes fresh in vitro fertilization/ intracytoplasmic sperm injection (IVF/ICSI) cycles. Semen quality deteriorates with increasing age; however, there is conflicting evidence for any impact may have outcome IVF/ICSI. Several retrospective and prospective cohort studies shown that increases miscar...

conclusions we found acceptable short-term survival in both groups; however, living donor recipients continue to have better long-term patient and graft survival rates. results although there were no significant differences in one-year survival rates of patient and graft between study groups, three-years survival rates of patient and graft were significantly longer in living donor kidney transp...

BACKGROUND The demand for sperm donors has continued despite the introduction of ICSI. This study was undertaken in the light of impending changes in donor anonymity laws to evaluate the recruitment process of sperm donors. METHODS Retrospective analysis of 1101 potential sperm donors in a tertiary referral centre between January 1994 and August 2003. The main outcome measures were to determi...

Modem forensic techniques allow DNA to be extracted from ever decreasing amounts of cellular material. Low copy number (LCN) profiling enables the production of STR profiles from small numbers of cells. Moreover, methods such as laser micro-dissection enables forensic scientists to potentially isolate individual cells for PCR. The DNA derived from haploid cells (semen) is a common source of for...