cyp3a4 probably has the broadest catalytic activity of any cytochrome p450. it is a crucial task to test new drug candidates in a reliable system for their ability to induce expression of this enzyme. firstly, a total of 300 bp core distal enhancer of cyp3a4 xrem region (-7972/-7673) were amplified from human genomic dna. the pcr product was then ligated into a human secretory alkaline phosphat...

The secretory IgA system was investigated in children with protein-calorie malnutrition (PCM). The results of the study indicated that in children suffering from kwashiorkor and marasmus the concentration of IgA in duodenal fluid, saliva, nasal secretions, and tears was significantly reduced on admission and returned to normal 4 weeks after treatment. However, the concentration of secretory IgA...

The p24alpha, -beta, -gamma, and -delta proteins are major multimeric constituents of cycling endoplasmic reticulum-Golgi transport vesicles and are thought to be involved in protein transport through the early secretory pathway. In this study, we targeted transgene overexpression of p24delta2 specifically to the Xenopus intermediate pituitary melanotrope cell that is involved in background ada...

The constituents of the secretory immunoglobulin A system (dimeric IgA, total secretory component and free secretory component) were measured in sputum sol phase, tracheal aspirates, and bronchoalveolar lavage fluids from 15 patients undergoing fibreoptic bronchoscopy. All of the proteins showed a progressive decrease in concentration from sputum to the bronchoalveolar lavage fluids (2p less th...

Core components of the secretory pathway have largely been identified and studied in single cell systems such as the budding yeast S. cerevisiae or in mammalian tissue culture. These studies provide details on the molecular functions of the secretory machinery; they fail, however, to provide insight into the role of these proteins in the context of specialized organs of higher eukaryotes. Here,...

Chromogranin A (CgA) is transported restrictedly to secretory granules in neuroendocrine cells. In addition to pHand Ca2 -dependent aggregation, CgA is known to bind to a number of vesicle matrix proteins. Because the binding-prone property of CgA with secretory proteins may be essential for its targeting to secretory granules, we screened its binding partner proteins using a yeast two-hybrid s...

Chromogranin A (CgA) is transported restrictedly to secretory granules in neuroendocrine cells. In addition to pH-and Ca 2 +-dependent aggregation, CgA is known to bind to a number of vesicle matrix proteins. Because the binding-prone property of CgA with secretory proteins may be essential for its targeting to secretory granules, we screened its binding partner proteins using a yeast two-hybri...

fascioliasis is a worldwide parasitic disease in human and domestic animals. the causative agents of fascioliasis are fasciola hepatica and fasciola gigantica. in the recent years, fasciola resistance to drugs has been reported in the many of publications. fasciola spp has detoxification system including gst enzyme which may be responsible for its resistance. therefore , the aim of the study wa...

Synaptic vesicle 2 (SV2) proteins, critical for proper nervous system function, are implicated in human epilepsy, yet little is known about their function. We demonstrate, using direct approaches, that loss of the major SV2 isoform in a central nervous system nerve terminal is associated with an elevation in both resting and evoked presynaptic Ca(2+) signals. This increase is essential for the ...