Reduced gastrointestinal HCO3- secretion contributes to malabsorption and obstructive syndromes in cystic fibrosis. The apical HCO3- transport pathways in these organs have not been defined. We therefore assessed the involvement of apical Cl-/HCO3- exchangers and anion conductances in basal and cAMP-stimulated duodenal HCO3- secretion. Muscle-stripped rat and rabbit proximal duodena were mounte...

Mice deficient for the apical membrane oxalate transporter SLC26A6 develop hyperoxalemia, hyperoxaluria, and calcium oxalate stones due to a defect in intestinal oxalate secretion. However, the nature of the basolateral membrane oxalate transport process that operates in series with SLC26A6 to mediate active oxalate secretion in the intestine remains unknown. Sulfate anion transporter-1 (Sat1 o...

The actions of vasoactive intestinal polypeptide (VIP) on catecholamine secretion and changes in [Ca2+]i in single rat chromaffin cells were studied using amperometry and Indo-1. Application of VIP prior to acetylcholine (ACh) or co-application of VIP and ACh enhanced secretion by 94% and 153% respectively, compared to ACh alone. [Ca2+]i was increased by 17% when VIP was preapplied and by 73% u...

Classification of Adrenal Cortical Disorders Lesions of the adrenals frequently cause disturbance of endocrine function. Various clinical syndromes may result, depending on whether the secretion is increased or diminished and, when it is increased, which hormones predominate. The lesions and syndromes may be classified as follows: A. WITH DISTURBANCE OF CORTICAL FUNCTION i. Increased Secretion:...

Tango1 enables ER-to-Golgi trafficking of large proteins. We show here that loss of Tango1, in addition to disrupting protein secretion and ER/Golgi morphology, causes ER stress and defects in cell shape. We find that the previously observed dependence of smaller cargos on Tango1 is a secondary effect. If large cargos like Dumpy, which we identify as a Tango1 cargo, are removed from the cell, n...

Dispersed pancreatic acini were studied to assess the function of junctional coupling between adult secretory cells. Nonstimulated control cells were extensively coupled to their neighbors throughout each acinus. Addition of heptanol caused their uncoupling and increased their basal amylase release. Neurotensin, secretin, and vasoactive intestinal peptide (VIP) stimulated amylase secretion with...

Classification of Adrenal Cortical Disorders Lesions of the adrenals frequently cause disturbance of endocrine function. Various clinical syndromes may result, depending on whether the secretion is increased or diminished and, when it is increased, which hormones predominate. The lesions and syndromes may be classified as follows: A. WITH DISTURBANCE OF CORTICAL FUNCTION i. Increased Secretion:...

We have examined the ability of Helianthus hypocotyl segments as well as segments from a variety of other species to elongate in response to H(+) and to secrete H(+) in response to auxin and fusicoccin. In all cases a positive response was obtained when the cuticular barrier was abraded with carborundum. Removal of the cuticular barrier by "peeling" prevented detection of both auxin-induced elo...

Drosophila CrebA facilitates high-level secretion by transcriptional upregulation of the protein components of the core secretory machinery. In CrebA mutant embryos, both salivary gland (SG) morphology and epidermal cuticle secretion are abnormal, phenotypes similar to those observed with mutations in core secretory pathway component genes. Here, we examine the cellular defects associated with ...

migratory birds can become long-distance vectors for a wide range of microorganisms. the objective of the present study was to investigate the presence of cryptococcus neoformans in the cloacae, crop and nasal cavity of migratory birds in order to assess their role as potential reservoirs and/or mechanical vectors of human and animal cryptococcosis. a total of 700 samples (cloacae, crops and na...