toxoplasma gondii contain various immunogenic antigens. the most important toxoplasma antigens are somatic and excreted/secreted antigens. rhoptry proteins are known as excreted/secreted antigens. these antigens have been proposed as a vaccine candidate against toxoplasmosis. the main objective of the present work was cloning rhoptry protein1 (rop1) gene of toxoplasma gondii (rh) in a cloning v...

During recent years, increased efforts have focused on elucidating the secretory function of skeletal muscle. Through secreted molecules, skeletal muscle affects local muscle biology in an auto/paracrine manner as well as having systemic effects on other tissues. Here we used a quantitative proteomics platform to investigate the factors secreted during the differentiation of murine C2C12 skelet...

The analysis of secreted proteins represents a challenge for current proteomics techniques. Proteins are usually secreted at low concentrations in the culture media, which makes their recovery difficult. In addition, culture media are rich in salts and other compounds interfering with most proteomics techniques, which makes selective precipitation of proteins almost mandatory for a correct subs...

The effector proteins of the type III secretion systems of many bacterial pathogens act in a coordinated manner to subvert host cells and facilitate the development and progression of disease. It is unclear whether interactions between the type-III-secreted proteins of Pseudomonas aeruginosa result in similar effects on the disease process. We have previously characterized the contributions to ...

The book Heat Shock Proteins (HSP) in Molecular and Cellular Biology of Human Diseases Volume 28 provides the most comprehensive up-to-date review on new exciting therapies that utilize mechanisms based heat shock response targeting various stress proteins as a promising therapeutic strategy for wide variety human diseases including all types cancers inflammatory diseases. Using an integrative ...

Expression of eukaryotic proteins in E. coli often results in their aggregation. Proper folding and solubility of therapeutical proteins are the pre-requisite for their bioactivity. This is not achieved in cytoplasmic expression in E. coli because of the absence of disulfide bonds formation. A novel expression/secretion vector was constructed which exploited β-lactamase signal sequence to trans...