Human colon tissue explant culture provides a physiologically relevant model system to study human gut biology. However, the small (20-30 mg) and complex tissue samples used present challenges for monitoring tissue stability, viability, and provision of sufficient tissue for analyses. Combining molecular profiling with explant culture has potential to overcome such limitations, permitting inter...

Background: Staphylococci are recognized worldwide as one of the most important etiological agents of bovine mastitis due to their virulence factors such as their ability to penetrate inside mammary epithelial cells and their ability to form biofilm. Aims: The objectives of this study were to establish a model of primary mammary epithelial cells origin...

An efficient procedure has been developed for callus induction and cell suspension cultures of C. saxicola for the first time. Explant selection was carried out among leaf, stem and root to select a suitable type of explants capable of higher callus formation. Leaf explants thus selected showed maximum response to callus induction (67.1%). Modified B5 medium supplemented with 0.5 mg l(-1) 2,4-D...

to produce embryogenic callus and somatic embryos, tendril explants were collected and cultured in ms medium supplemented with various concentrations of hormones. after one month most of explants produced callus. the highest percent of embryogenic callus obtained in ms medium supplemented with 4.5 µm bap and 1µm 2,4-d. production of somatic embryos was depended on genotype and medium. yaghouti ...

Automation of cell culture would facilitate stable cell expansion with consistent quality. In the present study, feasibility of an automated closed-cell culture system "P 4C S" for an embryoid body- (EB-) explant outgrowth culture was investigated as a model case for explant culture. After placing the induced pluripotent stem cell- (iPSC-) derived EBs into the system, the EBs successfully adher...

Explant of trigeminal ganglia neurons in adult rats induces perineuronal glial proliferation of primarily satellite cells as opposed to Schwann cells. This proliferation begins at 15 h after explant culture and by 27 h there is a significant increase in glial proliferation as measured by scintillation counts of [3H]thymidine. Blocking protein synthesis between 0 and 3.5 h after explant culture ...

Several studies have shown that decidual tissue produces soluble factors which can mediate a variety of immunosuppressive activities. In order to assess whether prostaglandin E2 (PGE2) was present in supernatants produced by explant culture of 10 human specimens of first trimester decidua. The effect of the supernatants from the two different series (with and without indomethacin treatment) of ...

Introduction Joint injuries increase the likelihood of developing post-traumatic osteoarthritis (PTOA) due to the initiation of catabolic processes that lead to progressive degeneration of the joint cartilage. Several in vitro models have been developed (using chondral explants) to investigate the pathologic mechanisms of PTOA and to evaluate the efficacy of putative small molecule therapeutics...

Ex vivo explant culture models are powerful tools in bone research. They allow investigation of bone and cartilage responses to specific stimuli in a controlled manner that closely mimics the in vivo processes. Because of limitations in obtaining healthy human bone samples the explant growth of animal tissue serves as a platform to study the complex physico-chemical properties of the bone. More...