We have identified the replication origin of pNRC100, a 200-kb plasmid of Halobacterium halobium, by assaying for replication ability of miniplasmids containing cloned fragments of pNRC100 and the mevinolin resistance selectable marker of Haloferax volcanii. First, we showed the replication ability of plasmid pNGHCMEV1, which contains the 19-kb HindIII-C fragment of pNRC100, by recovery of plas...

Bacterial DNA-methyltransferases are responsible for site-specific modification of DNA. They transfer the methyl-group from Sadenosylmethionine (SAM) to DNA with formation of N6-methyladenosine (m6A), 5-methylcytosine (m5C) or N4-methylcytosine (m4Q (1). Lately nucleotide structures of more than one hundred genes of different methyltransferases have been determined (2). Analysis of deduced amin...

Applications in Plant Sciences Viburnum L. (Adoxaceae) is a clade of approximately 165 species of shrubs and small trees that occur in northern temperate forests, the mountains of Central and South America, and subtropical montane forests of Southeast Asia. The phylogeny of Viburnum provides a clear understanding of relationships among major clades (Spriggs et al., 2015). However, evolutionary ...

The isolation of genes from a given genomic region can be a rate-limiting step in the discovery of disease genes. We describe an approach to the isolation of cDNAs that have sequences in common with large genomic clones such as bacterial artificial chromosomes. We applied this method to loci both amplified and deleted in cancer, illustrating its usage in the identification of both oncogenes and...

UNLABELLED African swine fever is one of the most devastating pig diseases, against which there is no vaccine available. Recent work from our laboratory has demonstrated the protective potential of DNA vaccines encoding three African swine fever viral antigens (p54, p30, and the hemagglutinin extracellular domain) fused to ubiquitin. Partial protection was afforded in the absence of detectable ...

We present a novel subtractive enrichment protocol for the identification of differentially expressed mRNA species. This procedure, called SABRE (selective amplification via biotinand restriction-mediated enrichment), uses selective streptavidin-biotin affinity and restriction enzyme site reconstitution to enrich for cDNA species more abundant in one population than in another. Analysis of live...

Plasmid-mediated CTX-M-type extended-spectrum -lactamases (ESBLs) are being increasingly reported worldwide in enterobacterial isolates (20). They may be grouped according to their amino acid identity in four clusters: CTX-M-1 (CTXM-1, -3, -10, -11, -12, -15, and -22), CTX-M-2 (CTX-M-2, -4, -5, -6, -7, and -20 and Toho-1), CTX-M-8, and CTX-M-9 (CTXM-9, -13, -14/-18, -16, -19, and -21 and Toho-2...

Mutations in the human Tsc1 and Tsc2 genes predispose to tuberous sclerosis complex (TSC), a disorder characterized by the wide spread of benign tumors. Tsc1 and Tsc2 proteins form a complex and serve as a GTPase-activating protein (GAP) for Rheb, a GTPase regulating a downstream kinase, mTOR. The genome of Schizosaccharomyces pombe contains tsc1 and tsc2, homologs of human Tsc1 and Tsc2, respe...