BACKGROUND Plasma phospholipid transfer protein (PLTP) plays a central role in the remodeling of HDLs. Reliable and accurate methods for assaying PLTP concentration are required. METHODS A sandwich ELISA for PLTP has been developed, using two monoclonal antibodies against recombinant human PLTP (rhPLTP) expressed in Chinese hamster ovary cells. The ELISA allows for the quantification of PLTP ...

Enzyme-linked immunosorbent assays (ELISA) for detecting malarial parasites in blood were tested on uninfected monkeys and in monkeys infected with Plasmodium falciparum. A double antibody sandwich ELISA detected one malarial cell per 10(3) uninfected cells, and an inhibition ELISA detected on infected cell per 10(4) uninfected erythrocytes. These methods are not yet as sensitive as conventiona...

Several systems for enzyme-linked immunosorbent assay (ELISA) of thermostable direct hemolysin (TDH) of Vibrio parahaemolyticus were tested, and single-antibody sandwich ELISA systems gave satisfactory results. ELISA was able to detect as little as several nanograms of purified TDH per milliliter. The method of De Jong (J. Clin. Microbiol. 17:928-930, 1983) and the glutaraldehyde method were su...

Hakkari ili domates ve biber üretim alanlarında lekeli solgunluk virüsü (Tomato spotted wilt virus, TSWV) yonca mozaik (Alfalfa mosaic AMV)’nün belirlenmesi için yapılan sürveyler 2020 döneminde gerçekleştirilmiştir. Domates sararma, nekroz, şekil bozukluğu simptomu gösteren 184 bitkiden yaprak örneği toplanmıştır. Toplanan örneklerinde TSWV AMV’nin Double antibody sandwich enzyme linked immuno...

Proteins having multiple epitopes can be usually measured with sandwich ELISA employing two kinds of antibodies, which permits high sensitivity as well as a wide working range of more than three orders of magnitude. On the other hand, so-called monovalent antigens with MW less than 1000 are not susceptible to sandwich assays due to their small size and have almost always been measured in compet...

We developed a novel silica coating magnetic nanoparticle-based silver enhancement immunoassay (SEIA) for ricin toxin (RT) rapid electrical detection using interdigitated array microelectrodes (IDAMs) as electrodes. This novel system was developed by taking advantage of the separation and enrichment properties of magnetic nanoparticles (MNPs) and the catalytic properties of gold nanoparticles (...

AIMS/INTRODUCTION The aims of the present study were to investigate the performance of a novel sandwich enzyme-linked immunosorbent assay (ELISA) for measuring glucagon (1-29) with monoclonal antibodies against both the C- and N-terminal regions of glucagon (1-29), and to analyze the differences in plasma levels and responses of glucagon (1-29) to oral glucose loading in normal glucose toleranc...

A series of tests were conducted to determine if the sensitivity and efficiency of an established rabbit immunoglobulin (IgG)-specific enzyme-linked immunosorbent assay (ELISA) could be improved for detecting a protein mark on insects. Five variations of ELISA were examined for their ability to detect a rabbit IgG mark on the convergent lady beetle, Hippodamia convergens Guérin-Méneville. The c...

β-Lactamase residues in milk represent a public health risk. The cylinder plate detection method, which is based on bacterial growth, is laborious and time consuming. In this study, 15 monoclonal antibodies (mAbs) were selected against Temoneira (TEM) 1 β-lactamase. A sandwich enzyme-linked immunosorbent assay (ELISA) based on an optimum mAb pair was developed and validated for the detection of...