The objectives of the studies were to determine if radiation and chlorine acted in a simple additive process or if a nonadditive increase in inactivation occurred when chlorination followed γ-irradiation both in vitro and in situ. Separate studies evaluated the effects of γ-irradiation (0.2 kGy at 20 ◦C), chlorination (0.5 ppm for 10 min), or irradiation followed by chlorination of Salmonella, ...

The aim of this study was to evaluate the survival of Salmonella, Escherichia coli O157:H7, Listeria monocytogenes, and Staphylococcus aureus on natural black olives during aerobic storage without brine and to conclude about the safety of this fermented food. Fermented black olives (pH 3.95; NaCl 6.02%) were artificially inoculated with 4 strains of Salmonella enterica ser. Enteritidis, 4 strai...

For economic, agricultural, and environmental reasons, composting is frequently used for organic waste recycling. One approach to limiting the potential risk from bacterial food-borne illnesses is to ensure that soil amendments and organic fertilizers are disinfected. However, more knowledge concerning the microbiological safety of composted substrates other than sludge and manure is necessary....

Stevia rebaudiana Bertoni, is an ancient perennial shrub of South America, with great potential as a crop for the production of high-power natural sweetener. Due to its chemical composition and content of the phytochemical compounds, it is suitable as a raw material for the extraction and production of functional foods. Diterpene glycosides component of low-calorie sweetener is approximately 30...

Microgreens are an emerging salad crop with properties similar to those of sprouted seeds and lettuce. This study aimed determine bacterial pathogen persistence during microgreen cultivation transfer from soil-free matrix (SFCM) mature microgreens. Salmonella enterica subsp. ser. Javiana Listeria monocytogenes were inoculated onto biostrate mats as well peat SFCM sampled (day 0). Next, sunflowe...

The efficacy of the water-assisted ultraviolet-C light (WUVC) strategy was evaluated as an alternative to chlorine sanitization and compared ‘conventional’ dry technology (DUVC) for inactivation Salmonella enterica, Listeria monocytogenes murine norovirus (MNV-1) on strawberries. Strawberries were washed in a laboratory scale prototype (LAB-UVC-Gama) consisting tank filled with water, equipped ...

With this study, 28 pools of snails of the genus Helix, respectively Helix aspersa (n=24) and Helix vermiculata (n=4) were analysed. They were taken from snail farming and stores. The snails were from Sardinia, other regions of Italy, and from abroad. All the samples were examined as pool looking for these microbiological target: Salmonella spp., Listeria monocytogenes, Escherichia coli O157, C...

The objective of this study was to determine the prevalence of Salmonella, Listeria monocytogenes, and fecal coliforms in bulk tank milk in the United States. As part of the NAHMS Dairy 2002 survey, 861 bulk tank milk samples were collected from farms in 21 states. Milk was directly plated on selective agars for direct bacterial enumeration and was enriched in selective broths to increase detec...

Various enteric bacterial pathogens target the host cell cytoskeletal machinery as a crucial event in their pathogenesis. Despite thorough studies detailing strategies microbes use to exploit these components of the host cell, the role of the spectrin-based cytoskeleton has been largely overlooked. Here we show that the spectrin cytoskeleton is a host system that is hijacked by adherent (Entrop...

This study evaluated the capacity of real-time PCR and conventional culture methods to detect Salmonella enterica serovar Typhimurium Listeria monocytogenes adhered stainless steel surfaces used as food contact surfaces. The adhesion microorganisms was performed under dry conditions represent stress which these pathogens can be subjected in processing environment. samples were analyzed with var...