The aim of the study was to evaluate the supplementation of active yeast (Saccharomyces cerevisiae) on diets of lambs fed with two different concentrate proportion on DM intake, apparent digestibility of nutrients, in vitro digestibility of dry matter and rumen fermentation parameters. Twenty non-castrated male lambs, averaging 120 days-old, housed in metabolism cages, with collector and separa...

The supernatant from centrifugation at 1,000 x g of strained rumen fluid was lyophilized, and the residue and sublimate fractions were used to replace fresh rumen fluid in a complete roll tube medium for enumeration of total rumen bacteria. Most of the growth-supporting nutrients in fresh rumen fluid were found in the residue fraction. With one exception, no significant differences were found i...

SARA is a common and serious problem in well-managed and intensive dairy herds or beef cattle operations, because of triggering other metabolic disorders and causing lactation-fertility losses. It is a metabolic disease in dairy cattle that occurs during early and mid-lactation and has traditionally been characterized by low rumen pH, but lactic acid does not accumulate as in acute lactic acid ...

Six adult castrated male red deer (Cervus elaphus), fitted with rumen cannulas, were offered chaffed lucerne hay ad lib. during winter and summer, with voluntary food intake (VFI) being respectively 59 and 89 g dry matter intake (DMI)/kg bodyweight 0.75 per d. The same animals were also offered the same feed during summer, with intake restricted to that of winter VFI. The apparent digestibility...

BACKGROUND Understanding the host impact on its symbiotic microbiota is important in redirecting the rumen microbiota and thus improving animal performance. The current study aimed to understand how rumen microbiota were altered and re-established after being emptied and receiving content from donor, thus to understand the impact of such process on rumen microbial fermentation and to explore th...

Protozoa population, microbial synthesis efficiency and rumen degradability of dry matter and neutral detergent fiber in cattle fed on diets with different lipid rates were evaluated. Nine 16-monthold Nelore young bulls, cannulated in the rumen and duodenum, weighing 232 ± 35 kg, were used in the trial. Experimental design consisted of a 3 x 3 square in triplicate, comprising the following trea...

AIM The present study was conducted to examine the rumen modulatory effect of thyme, clove and peppermint oils on rumen fermentation pattern in vitro using roughage based diet. MATERIALS AND METHODS Thyme, clove and peppermint oils were tested at concentration of 0, 30, 300 and 600 mg/l (ppm) of total culture fluid using in vitro gas production technique in wheat straw based diet (concentrate...

The comparison of the bacterial profile of intracellular (iDNA) and extracellular DNA (eDNA) isolated from cow rumen content stored under different conditions was conducted. The influence of rumen fluid treatment (cheesecloth squeezed, centrifuged, filtered), storage temperature (RT, -80 °C) and cryoprotectants (PBS-glycerol, ethanol) on quality and quantity parameters of extracted DNA was eval...

Background: Aflatoxins are fungal secondary metabolites negatively affecting ruminant performance; however, little information is available on their impact on rumen fermentation. Aims: This study aimed at determining the effects of different concentrations of aflatoxin B1 (AFB1) from Aspergillus flavus on in vitro gas production and ruminal fe...

Background: This study was carried out to evaluate the effect of supplementing ruminant ration with propionibacterium P169 by using 3X3 Latin square Rahmani rams on feed intake, nutrients digestibility, rumen fermentation, blood plasma metabolites. Methods: Rams were divided into three groups with three successive experimental periods. Each period consisted of 21 days. Animals were fed on: rati...