In order to study the extent to which rumen soluble nitrogen can contribute to the intestinal flow, a study was carried out to simultaneously assess the dynamics of protein disappearance from dacron bags placed in the rumen and the amount of various N products in the rumen fluid (total nitrogen (tN), ammonia nitrogen (NH3-N), non-ammonia nitrogen (NAN)). The measurements were carried out on 4 s...

The present study was conducted to investigate the possibility of using a modified in vitro gas production technique in place of the nylon bag method to estimate protein degradability of alfalfa hay in ruminants. In the in situ experiment dacron bags were filled with 3 g alfalfa hay. This was incubated in the rumen of three ruminally cannulated Ghezel rams for the periods of 0, 2, 4, 8, 12, 16,...

1. A method for estimating the proportions of bacterial- and protozoal-N in the total non-ammonia-N reaching the lower gut of the ruminant under steady-state conditions was evaluated. Three trials using two different diets were conducted with a Holstein steer equipped with a rumen cannula and duodenal re-entrant cannulas. 2. An intraruminal primed infusion of (15NH4)2SO4 was administered for 68...

Experiments were carried out in vivo to investigate the pathways of ammonia incorporation into rumen bacteria, bacterial fractions and free amino acids within the bacteria. Steers were alternately given two isoenergetic, isonitrogenous diets containing the nitrogen mainly as either urea or decorticated groundnut meal (DCGM). At the end of each period on a given diet, a solution of 15NH4Cl was i...

Impact of feed efficiency and diet on adaptive variations in the bacterial community in the rumen fluid of cattle Assessment of the microbial ecology of luminal methanogens in cattle with different feed efficiencies Characterization of variation in rumen methanogenic communities under different dietary and host feed efficiency conditions, as determined by PCR-Denaturing Gradient Gel Electrophor...

the present study was conducted to investigate the possibility of using a modified in vitro gas production technique in place of the nylon bag method to estimate protein degradability of alfalfa hay in ruminants. in the in situ experiment dacron bags were filled with 3 g alfalfa hay. this was incubated in the rumen of three ruminally cannulated ghezel rams for the periods of 0, 2, 4, 8, 12, 16,...

This experiment was designed to investigate the effect of lerak extract on the dynamic of rumen microbes in the in vitro fermentation of diet with different ratios of forage and concentrate. In vitro fermentation was conducted according to the method of Tilley and Terry (1963). The design of experiment was a factorial block design with 2 factors. The first factor was the ratio of forage and con...

We used 16S rRNA gene sequencing to examine the posteffects of antibiotic treatment on the structure and metabolism of rumen microbiota. Twelve goats were randomly assigned into two groups, with one group receiving intramuscular streptomycin injection at 40 mg/kg bodyweight daily for 10 days. At 4 weeks after treatment with antibiotic, three goats were randomly selected from each group and swit...