AIMS To determine whether lactic acid bacteria (LAB) used in inoculants for silage can survive in rumen fluid (RF), and to identify those that survive best. METHODS AND RESULTS Twelve commercial silage inoculants were added at 107 CFU ml-1 to strained RF (SRF) taken from dairy cows, with and without 5 g l-1 glucose and incubated in vitro at 39 degrees C. Changes in pH, LAB numbers and ferment...

the objective of this study was to evaluate the effects of supplementation of different level protein diets with rumen protected methionine and lysine on performance and ruminal parameters in holstein dairy cows when in their early lactation. twenty one dairy cows in their early lactations were assigned to three treatments in a randomized block design experiment of a 35-d duration period. diets...

A growth medium and test were developed for rapid detection of urease in fermentative anaerobic bacteria. Using nonselective rumen fluid roll-tube agar medium and the new test, it was confirmed that Peptostreptococcus productus is often the most numerous urease-forming species in human feces. Also, some fecal strains of Ruminococcus albus, Clostridium innocuum, and Clostridium beijerinckii prod...

The objective of this study was to determine the effect of dietary concentration of dl-malic acid (MA) on DMI, CH(4) emission, and rumen fermentation in beef cattle. Two Latin square experiments were conducted. In Exp. 1, six beef heifers (19 +/- 1 mo old) were assigned in a duplicated Latin square to 1 of 3 dietary concentrations of MA on a DMI basis (0%, MA-0; 3.75%, MA-3.75; or 7.5%, MA-7.5)...

This study was conducted to investigate the effects of recycled poultry bedding (RPB) treated with different levels of pomegranate peel extract (PPE) as a tannin source on cellulolytic bacterial population and rumen fermentation parameters of fattening lambs. For this purpose, twenty-eight Arabian lambs (19.70 ± 2.45 kg body weight, 90 ± 12 days of age) were randomly assigned to four dietary tr...

INTRODUCTION We have reported that altered gut flora is associated with septic complications and eventual death in critically ill patients with systemic inflammatory response syndrome. It is unclear how fecal pH affects these patients. We sought to determine whether fecal pH can be used as an assessment tool for the clinical course of critically ill patients. METHODS Four hundred ninety-one f...

The aim of this study was to investigate the effect of two different levels of starter protein and amounts of milk fed on animal health, fecal score, immune responses and microbial population in the rumen of Holstein male calves. Two levels of starter crude protein (17 and 20% of starter dry matter (DM) and two levels of milk fed (7 and 10% of body weight) were combined in a 2 × 2 factorial exp...

Using 24 hours in vitro cultures of rumen microorganisms, this study investigates the effect of buffering capacity of 2 inorganic compounds (M1=119.43 and M2=116.50 meq/L) on the in vitro rumen acidogenic value (AV), medium pH, dry matter disappearance (INVDMD) and methane emission of lactating dairy cow diets containing various forage to concentrate ratios as 40:60 (FC40:60) and 30:70 (FC30:70...

Binding of IgG antibodies to Entodinium spp. in the rumen of sheep (Ovis aries) was investigated by adding IgG, purified from plasma, directly into the rumen. Plasma IgG was sourced from sheep that had or had not been immunized with a vaccine containing whole fixed Entodinium spp. cells. Ruminal fluid was sampled approximately 2 h after each antibody dosing. Binding of protozoa by a specific an...

I . Details are given for the construction and use of an apparatus for the incubation of rumen liquor in semi-permeable sacs inside the bovine rumen. It was found practicable, using one animal, to incubate simultaneously samples of strained diluted rumen liquor in three identical vessels, each of 100--120 ml capacity, for periods of up to 23 h. 2. The optimal conditions for experiments of this ...