نتایج جستجو برای: rt lamp
تعداد نتایج: 70522 فیلتر نتایج به سال:
RT-LAMP法を用いて，ナシ（Pyrus spp.）の葉からナシ葉退緑斑点随伴ウイルス（PCLSaV）を検出する技術を開発した。設計したプライマーセットのうち，R2LP4が最も増幅が早く，退緑斑点症状のあるナシ葉の全RNAから15分以内にPCLSaVが検出された。12県で採取されたニホンナシおよびセイヨウナシの葉を用いた診断では，RT-LAMP法は既報のRT-PCR法と同等の感度と精度を示した。さらに，注射針で採取した汁液を用いた簡易なRT-LAMP検定においてもPCLSaVを検出することができた。また，塩基配列情報の比較から，設計したプライマーの一部は，日本以外で発生が確認されているPCLSaVの中国分離株と適合する可能性が示された。
We demonstrate an integrated microneedle (MN)-smartphone nucleic acid amplification platform for “sample-to-answer” diagnosis of multiplexed plant pathogens within 30 min. This portable system consists a polymeric MN patch rapid extraction minute and 3D-printed smartphone imaging device loop-mediated isothermal (LAMP) reaction detection. expanded the technology DNA targets as in previous study ...
BACKGROUND There is an increasing need for quantitative technologies suitable for molecular detection in a variety of settings for applications including food traceability and monitoring of genetically modified (GM) crops and their products through the food processing chain. Conventional molecular diagnostics utilising real-time polymerase chain reaction (RT-PCR) and fluorescence-based determin...
Background and purpose: Exosome as drug delivery system is a novel and smart methodology enabling delivery of exosome cargo into specific tissue. This aim could be accessed by manipulation of exosome producer cells for expression of specific transmembrane-anchored ligand on exosomes surface. Accordingly, Lysosomal Associated Membrane Protein (LAMP) is one of the best choices for anchoring and c...
A cDNA encoding the chicken orthologue of dendritic cell-lysosomal associated membrane protein (DC-LAMP)/CD208 was cloned by RT-PCR from RNA isolated from mature chicken bone marrow-derived dendritic cells (chBM-DCs). The cloned chicken DC-LAMP (chDC-LAMP) cDNA consists of 1281 nucleotides encoding an open reading frame of 426 amino acids (aa). Comparison of the deduced aa sequence of DC-LAMP w...
We developed a one-step, single-tube genogroup-specific reverse transcription–loop-mediated isothermal amplification (RT-LAMP) assay for the detection of group B Coxsackie genomes targeting 5’ UTR region. The amplification can be obtained in less than 1 h by incubating all of the reagents in a single tube with reverse transcriptase and Bst DNA polymerase at 63°C. Detection of gene amplification...
Abbreviations: Adeno-associated virus, (AAV); bacilli Calmette-Guerin, (BCG); base pairs, (bp); cottontail rabbit papilloma virus, (CRPV); E6-associated protein, (E6-AP); epidermal growth factor receptor, (EGFR); histone deacetylases, (HDAC); human papillomavirus, (HPV); inverted terminal repeats, (ITRs); listerolysin O, (LLO); of lysosome-associated membrane protein, (LAMP); open reading frame...
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