The in vitro phenotype of bovine articular chondrocytes is described. Chondrocytes plated at high density in roller-bottle and dish cultures were maintained in vitro. The major matrix macromolecules, collagen and proteoglycan, synthesized by these cells were characterized during the course of the culture period. The chondrocytes synthesized mainly Type II collagen, which was found predominantly...

The Fv fragment of the antibody B72.3 has been produced by expression in both a mammalian and microbial system, namely Chinese hamster ovary (CHO) cells and Escherichia coli. In both cases secretion of the Fv into the culture medium was achieved, with equivalent amounts of Vh and Vl produced. The yield of Fv from CHO cells was 4 mg/l in roller-bottle culture. E. coli proved to be a more product...

The purpose of this study was to compare roller pump and centrifugal pump technologies in routine adult open-heart operations to demonstrate the usefulness of applying a proven method of uniform risk stratification (outcomes research). Objective and readily available preoperative patient data was collected retrospectively on 102 non-randomized and consecutive adult open-heart operations. Group ...

A hydraulic jump is the sudden transition from a high-velocity impinging flow into a turbulent roller in an open channel. Substantial amounts of air are entrapped at the impingement point, and significant free-surface fluctuations take place above the roller. In the present study, some physical modelling was conducted in a relatively large sized facility. The flow conditions included a wide ran...

The purpose of this study was to investigate whether eight weeks of aerobic high-intensity interval training with roller ski is effective in cross-country skiers. 10 male (age, 18.28 ± 2.1 years; height, 171.26 ± 4.12 cm; weight 61.39 ± 6.28 kg) and 8 female (age, 16.05 ± 0.3 years; height, 158.3 ± 6.47 cm; weight, 49.34 ± 0.7 kg) junior cross country skiers completed the study. All skiers perf...

Adeno-associated viral (AAV) vectors are used for in vivo gene transfer in a number of preclinical models of genetic diseases (including large-animal models) and are currently being tested in clinical trials for treatment of hemophilia B and cystic fibrosis. Protocols for production of AAV vectors in a helper virus-free system are available and are based on transient transfection of HEK-293 cel...

BACKGROUND The purpose of this study is to evaluate the number of eyedrops available per bottle of a variety of commonly prescribed glaucoma medications. METHODS Six bottles of each glaucoma medication were tested: three each in the vertical and horizontal orientations. Bottles were housed in a customized force gauge apparatus designed to mimic ballpoint fingertip contact with a bottle. At a ...

OBJECTIVES To examine skaters' beliefs about risk factors related to roller derby injuries. DESIGN Qualitative study based on semistructured focus group discussions with female roller derby skaters. METHODS Skaters with varying levels of experience and injury history were invited to participate in focus group discussions. Semistructured focus group discussions were based on the question 'Wh...

We developed and validated a mitral valve orifice method for Doppler cardiac output determination. In 15 open-chest dogs, cardiac output was controlled and measured by a roller pump interposed between the right atrium and pulmonary artery as a right-heart bypass. Left heart flows were measured in the open-chest dog model by Doppler measurements at the mitral valve orifice and compared not only ...

In a comparison of 1,368 positive blood cultures, a vented Roche Septi-Chek (V-RSC) blood culture bottle was superior to an unvented tryptic soy broth-containing bottle (Difco) for the recovery of all aerobic and facultatively anaerobic microorganisms. Anaerobic bacteria were recovered more frequently and earlier in the unvented tryptic soy broth-containing bottle. A separate comparison of 529 ...