نتایج جستجو برای: rna purification
تعداد نتایج: 304817 فیلتر نتایج به سال:
Long noncoding RNAs are key regulators of chromatin states for important biological processes such as dosage compensation, imprinting, and developmental gene expression (1,2,3,4,5,6,7). The recent discovery of thousands of lncRNAs in association with specific chromatin modification complexes, such as Polycomb Repressive Complex 2 (PRC2) that mediates histone H3 lysine 27 trimethylation (H3K27me...
We have purified and characterized a DNA-dependent RNA polymerase from the blue-green alga Fremyela dipsiphon. This enzyme, purified by gel filtration, DEAE-ceflulose chromatography, and glycerol gradient centrifugation, is comprised of five polypeptide subunits. Their masses are 161,000, 134,000, 91,000, 72,000, and 41,000 daltons. Preparative electrophoresis of the purified enzyme on nondenat...
Glutamyl ribonucleic acid synthetase was purified approximately 150-fold from homogenates of rat liver to a specific activity of 60 mpmoles of glutamyl-RNA formed per min per mg of protein. The enzyme at this stage of purification was judged to be about 40% pure, but in a state of aggregation. Although the enzyme was quite stable to storage, it was very heat labile, and lost activity during its...
Sindbis 42 S genome RNA was isolated from virions and translated in vitro before and after purification by oligo(dT)-cellulose chromatography and sucrose density gradient centrifugation under denaturing conditions. In intact 42 S RNA, only the 5'-proximal initiation site for the synthesis of nonstructural proteins was used. The internally located start site for viral structural protein formatio...
Humans express four distinct non-protein-coding Y RNAs (ncRNAs). To investigate Y RNA functional diversification, we exploited an RNA-based affinity purification method to isolate ribonucleoproteins (RNPs) assembled on individual human Y RNAs. Silver staining and mass spectrometry revealed that the Ro and La proteins assemble with all Y RNAs, while additional proteins associate with specific Y ...
We demonstrate the first integrated microfluidic tmRNA purification and nucleic acid sequence-based amplification (NASBA) device incorporating real-time detection. The real-time amplification and detection step produces pathogen-specific response in < 3 min from the chip-purified RNA from 100 lysed bacteria. On-chip RNA purification uses a new silica bead immobilization method. On-chip amplific...
In Drosophila oocytes and neuroblasts, the double-stranded RNA binding protein Staufen assembles into ribonucleoprotein particles, which mediate cytoplasmic mRNA trafficking and translation. Two different mammalian orthologues also appear to reside in distinct RNA-containing particles. To date, relatively little is known about the molecular composition of Staufen-containing ribonucleoprotein co...
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