نتایج جستجو برای: rk39 antigen

تعداد نتایج: 200325  

مقدمه لیشمانیوز احشایی(کالاآزار) مدیترانه ای به عنوان یک بیماری اندمیک در میان کودکان استان خراسان شمالی شناخته شده است. روش تشخیصی سرولوژیک حساس و کم تهاجمی است. این مطالعه با هدف تعیین سرو اپیدمیولوژی موارد بدون علامت ، در مناطق آندمیک خراسان انجام شد. روش کار بررسی حاضر یک مطالعه توصیفی مقطعی از نوع کاربردی است که روی 442 کودک روستایی در سال 1392-1394 در دو استان خراسان رضوی و خراسان شمالی ا...

2017
Manish Sharma

The diagnosis of visceral leishmaniasis involves isolation of the parasites in culture or invasive and risky techniques including demonstration of parasite in stained preparations of relevant tissues such as spleen, bone marrow or lymph node by light microscopic examination remains a gold standard. Serological tests using rK39 in ELISA or rapid immunochromatographic tests, direct agglutination ...

Journal: :Clinical and diagnostic laboratory immunology 2005
K M Kurkjian L E Vaz R Haque C Cetre-Sossah S Akhter S Roy F Steurer J Amann M Ali R Chowdhury Y Wagatsuma J Williamson S Crawford R F Breiman J H Maguire C Bern W E Secor

Several serology-based immunoassays are used to diagnose visceral leishmaniasis (VL), a chronic protozoan parasitic disease caused by the Leishmania donovani complex. These tests are primarily designed to diagnose the most severe clinical form of VL, known as kala-azar. However, leishmanial infection is frequently asymptomatic and may manifest only as a positive serologic response or positive l...

Journal: :Revista da Sociedade Brasileira de Medicina Tropical 2017
Ivete Lopes de Mendonça Joilson Ferreira Batista Guilherme Loureiro Werneck Maria Regiane Araújo Soares Dorcas Lamounier Costa Carlos Henrique Nery Costa

INTRODUCTION The control of reservoirs for Leishmania infantum -induced zoonotic visceral leishmaniasis requires the identification of dogs posing a population risk. Here, we assessed the performance of several assays to identify Lutzomyia longipalpis infectious dogs. METHODS We evaluated 99 dogs that were positive for visceral leishmaniasis based on parasite identification. Serological analy...

2017
Getachew Ferede Ermias Diro Sisay Getie Gebeyaw Getnet Yegnasew Takele Anteneh Amsalu Yitayih Wondimeneh

BACKGROUND Despite high prevalence of visceral leishmaniasis and malaria in the study area, their coinfection remains unknown. Therefore, this study was aimed to document VL-malaria coinfections and their associated factors. METHODS A cross-sectional study was conducted among clinical suspected VL patients attending Metema hospital, Northwest Ethiopia, from January 2014 to June 2014. Blood sa...

2013
Emily R. Adams Inge Versteeg Mariska M. G. Leeflang

Identification of post-kala-azar dermal leishmaniasis (PKDL) is important due to the long and toxic treatment and the fact that PKDL patients may serve as a reservoir for visceral leishmaniasis (VL). We summarized the published literature about the accuracy of diagnostic tests for PKDL. We searched Medline for eligible studies investigating the diagnostic accuracy of any test for PKDL. Study qu...

2012
Jane Cunningham Epco Hasker Pradeep Das Sayda El Safi Hiro Goto Dinesh Mondal Margaret Mbuchi Maowia Mukhtar Ana Rabello Suman Rijal Shyam Sundar Monique Wasunna Emily Adams Joris Menten Rosanna Peeling Marleen Boelaert

BACKGROUND Poor access to diagnosis stymies control of visceral leishmaniasis (VL). Antibody-detecting rapid diagnostic tests (RDTs) can be performed in peripheral health settings. However, there are many brands available and published reports of variable accuracy. METHODS Commercial VL RDTs containing bound rK39 or rKE16 antigen were evaluated using archived human sera from confirmed VL case...

Journal: :Infection and immunity 2006
Yasuyuki Goto Rhea N Coler Jeffrey Guderian Raodoh Mohamath Steven G Reed

Visceral leishmaniasis (VL) is a form of leishmaniasis, which is caused by infection with the protozoan parasite Leishmania, and is often fatal unless it is treated. Rapid and accurate diagnosis of VL is important for effective treatment. Here we report the cloning of previously undescribed tandem repeat (TR) proteins of Leishmania infantum and an evaluation of VL patient antibody responses to ...

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