Schlenk, F. (Argonne National Laboratory, Argonne, Ill.), and J. L. Dainko. Action of ribonuclease preparations on viable yeast cells and spheroplasts. J. Bacteriol. 89:428-436. 1965.-Recrystallized pancreatic ribonuclease releases more than half of the ultraviolet-absorbing cellular constituents of Candida utilis and Saccharomyces cerevisiae. The presence of salts and buffer interferes with th...

In a continuation of the structural studies on ribonuclease reported in earlier papers (l-3), the products resulting from the action of pepsin on performic acid-oxidized ribonuclease have been examined by means of ion exchange chromatography. Eight peptides have been isolated in purified form and their amino acid compositions have been determined. In a following communication (4), a partial str...

The gene coding for the RNA subunit of ribonuclease P (RNase P) is essential in all free-living organisms. The RNA subunit, itself, is an enzyme and, from its evolutionary tree, we can infer that it is a very ancient molecule. The specificity of this enzyme is that it cleaves other RNA molecules at the junction of single-stranded and the 5' end of double-stranded regions of RNA. One can specula...

Peptide N-glycosidase from Flavobacterium meningosepticum cleaves complex as well as neutral glycoproteins (Plummer, T.H., Jr., Elder, J.H., Alexander, S., Phelan, A.W., and Tarentino, A.L. (1984) J. Biol. Chem. 259, 10700-10704). Examples of neutral glycoprotein substrates include ribonuclease B (one high mannose oligosaccharide chain) and yeast external invertase (nine chains/invertase subuni...

Similar differences were also found to exist in the ribonuclease hydrolysis of the two subunits of bacterial ribosomes (Godson & Cox, 1970). The different response of the subunits to hydrolysis by ribonuclease may indicate differences in the structural organization of the subunits. The electrophoretic pattern of RNA obtained from ribonuclease-treated subunits is shown in Fig. 2. The results ind...

The exposure of ribonuclease A to trichloroacetic acid was earlier shown to alter the conformation of the protein resulting in reduced enzymatic activity (Sagar, A. J., Subbiah, V., and Pandit, M. W. (1989) Biochim. Biophys. Acta 995, 144-150). We have studied the structure and enzymatic activity of ribonuclease A treated with trichloroacetic acid over a wide range of acid concentrations (0-40%...

Tomato bushy stunt virus (TBSV) and other tombusviruses encode a p19 protein (P19), which is a suppressor of RNAi. Wild-type TBSV or p19-defective mutants initially show a similar infection course in Nicotiana benthamiana, but the absence of an active P19 results in viral RNA degradation followed by recovery from infection. P19 homodimers sequester 21-nt virus-derived duplex siRNAs, and it is t...