PCR primers for diagnosis of Rhynchosporium secalis in seed samples of barley were developed. For the quantification of the pathogen in seed samples a real-time PCR with SYBR Green approach was used. Amounts from 1.8 to 419.1 pg of R. secalis DNA per 100 ng of total DNA were detected in 18 samples of barley seeds contaminated by R. secalis in field conditions. The correctness of this quantitati...

The effector protein NIP1 from the barley (Hordeum vulgare) pathogen Rhynchosporium secalis specifically induces the synthesis of defense-related proteins in cultivars of barley expressing the complementary resistance gene, Rrs1. In addition, it stimulates the activity of the barley plasma membrane H(+)-ATPase in a genotype-unspecific manner and it induces necrotic lesions in leaf tissues of ba...

To predict the response of plant pathogens to climate warming, data are needed on current thermal adaptation, the pathogen's evolutionary potential, and the link between them. We conducted a common garden experiment using isolates of the fungal pathogen Rhynchosporium commune from nine barley populations representing climatically diverse locations. Clonal replicates of 126 genetically distinct ...

Leaf blotch is a globally important disease of barley crops and other grasses that is caused by at least five host-specialised species in the fungal genus Rhynchosporium. The pathogen R. commune (specialised to barley, brome-grass and Italian ryegrass) has long been considered to reproduce only by asexual means, but there has been accumulating evidence for recombination and gene flow from popul...

Abstract Breeding of multi-resistant varieties to reduce yield loss due disease damage and lodging, reduction input intensity are high importance for a more sustainable cereal production. The aim this study was evaluate (i) caused by diseases lodging (ii) impact soil fertility in variety trials grown under two intensities. Intensity 2 treated with 1 without fungicides growth regulators. We appl...