The in vivo efficacy of glycosylated and nonglycosylated recombinant human granulocyte macrophage colony-stimulating factor (rh GM-CSF) expressed in Chinese hamster ovary cells and Escherichia coli respectively was studied in rhesus monkeys following a daily subcutaneous (SC; three times) or intravenous (IV; over six hours) dose for seven consecutive days. The monkeys responded to the rh GM-CSF...

Background Natural polysaccharides such as chitosan (CS) are widely used as antimicrobial agents. In recent years, and considering that CS has a strong antimicrobial potential, interest has been focused on antimicrobial activity of chitosan nanoparticles (CS NPs). The main factors affecting the antibacterial activity of chitosan include molecular weight (MW) and concentration. In this regard, t...

This research investigated the effect of temperature and relative humidity (RH) conditions during ethylene oxide (EtO) fumigation for inactivation Salmonella Enterococcus faecium NRRL B-2354 on whole black peppercorns. Black peppercorn samples were inoculated with a five-strain cocktail or E. inoculum. EtO (735.3 mg/L) was conducted at different treatment temperatures (46, 53, 60 °C) RH levels ...

The Mycobacterium tuberculosis stress response factor SigH plays a crucial role in modulating the pathogen's response to heat, oxidative-stress, envelope damage and hypoxia. We hypothesized that the lack of this key stress response factor would alter the interaction between the pathogen and its host cells. We compared the interaction of Mtb, Mtb:Δ-sigH and a strain where the mutation had been g...

A timber-concrete composite (TCC) slab composed of nail-laminated timber (NLT) and topping concrete (TC) was developed for flooring applications. The NLT laminated alternately with lumber plywood. To investigate the nonstructural dimensional behavior TCC slab, temperature, relative humidity (RH), changes exposed to outdoor air were monitored 205 days. Temperature change transmitted directly bot...

Leukotoxin (Ltx) is a prominent virulence factor produced by Aggregatibacter actinomycetemcomitans, an oral microorganism highly associated with aggressive periodontitis. Ltx compromises host responsiveness by altering the viability of neutrophils, lymphocytes, and macrophages. Previously, we developed a Rhesus (Rh) monkey colonization model designed to determine the effect of virulence gene mu...

We studied the pathology of acute toxoplasmosis in experimental mice inoculated with RH strain tachyzoites of Toxoplasma gondii. All died from severe disseminated toxoplasmosis involving the liver, spleen and pancreas. Pathological features of acute toxoplasmosis in susceptible mice could be regarded as an excellent model for acute reactivation of Toxoplasma in the immunosuppressed host.