نتایج جستجو برای: reverse genetics

تعداد نتایج: 183433  

Journal: :Proceedings of the National Academy of Sciences of the United States of America 2011
Shibani Mukherjee Eduardo J Firpo Yang Wang James M Roberts

The canonical function of the human telomerase protein (hTERT) is to synthesize telomeric DNA, but it has other biological activities, including enhancing cell proliferation, decreasing apoptosis, regulating DNA damage responses, and increasing cellular proliferative lifespan. The mechanistic relationships among these activities are not understood. We previously demonstrated that ectopic hTERT ...

Journal: :The Plant cell 2002
Allen Sessions Ellen Burke Gernot Presting George Aux John McElver David Patton Bob Dietrich Patrick Ho Johana Bacwaden Cynthia Ko Joseph D Clarke David Cotton David Bullis Jennifer Snell Trini Miguel Don Hutchison Bill Kimmerly Theresa Mitzel Fumiaki Katagiri Jane Glazebrook Marc Law Stephen A Goff

A collection of Arabidopsis lines with T-DNA insertions in known sites was generated to increase the efficiency of functional genomics. A high-throughput modified thermal asymmetric interlaced (TAIL)-PCR protocol was developed and used to amplify DNA fragments flanking the T-DNA left borders from approximately 100000 transformed lines. A total of 85108 TAIL-PCR products from 52964 T-DNA lines w...

2015
Ahmed Mostafa Pumaree Kanrai Henning Petersen Sherif Ibrahim Silke Rautenschlein Stephan Pleschka

Influenza A viruses (IAVs) are the most relevant and continual source of severe infectious respiratory complications in humans and different animal species, especially poultry. Therefore, an efficient vaccination that elicits protective and neutralizing antibodies against the viral hemagglutinin (HA) and neuraminidase (NA) is an important strategy to counter annual epidemics or occasional pande...

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