Background and objectives: ZatariamultifloraBioss. (ZM) has shown several effects in gastrointestinal, respiratory and nervous system. In this study, the effect of Z. multiflora was examined in colon cancer cell lines of Caco-2. Methods: Total extract and petroleum ether, chloroform and methanol fractions of the plant were prepared us...

A quantitative ultrastructural radioautographic study of in vitro protein synthesis has been carried out in rat synaptosomal fractions incubated with tritiated leucine or a tritiated amino acid mixture. Analysis of grain density distribution demonstrated that presynaptic endings are labeled. 30-50% of the developed grains, representing tritiated amino acids incorporated into proteins, were rela...

Chlorella vulgaris has been recognized as an interesting alternative feeding source since it contains a good amount of high-quality protein. However, the presence recalcitrant cell wall strongly affects nutrients’ digestibility, bioaccessibility, and bioavailability. The present study aimed to determine influence different pre-treatments (bead milling, extrusion, freeze-drying, heating, microwa...

BACKGROUND The objective of the present study was to isolate and purify the protein fraction(s) of llama seminal plasma responsible for the ovulation-inducing effect of the ejaculate. METHODS Semen collected from male llamas by artificial vagina was centrifuged and the seminal plasma was harvested and stored frozen. Seminal plasma was thawed and loaded onto a Type 1 macro-prep ceramic hydroxy...

Blood serum has been extensively explored as a source of the bio-markers [1, 2].  For concentration of minor protein(s) and depletion of abundant blood serum proteins a 2,2,2-trichloroacetic acid (TCA) precipitation procedure is frequently applied [3]. However, a significant amount of proteins may be present in the TCA extracts, and these proteins are often not studied.Recently, we have sh...

The colloid or protein osmotic pressure (Pi) is a function of protein molarity (linear) and of Donnan and other effects. Albumin is the major osmotic protein, but also globulins influence Pi. Equations based on concentrations of albumin and nonalbumin (globulin concentration + fibrinogen concentration) protein approximate Pi better than albumin alone. Globulins have a wide range of molecular we...

Ninety-six patients were observed at intervals for as long as three and one-half years in some cases. In most instances multiple determinations were made during this time using the electrophoretic pattern of the proteins before and after cold ether extraction (McFarlane method), to measure lipid transport. Hyperlipemia usually produced an increased in beta globulin. A varying decrease in albumi...