Studies in molecular and genetic epidemiology require a high-throughput, low cost, and reliable means of genomic DNA collection. Buccal (cheek) swabs have been proposed as a means of achieving these goals, but there is little information about the practical application of this approach. From January 1995 to December 1997, we processed 995 buccal swabs for use in polymerase chain reaction (PCR)-...

The aim of this study was to analyze 22 cytokine polymorphisms in the Roma population from the Republic of Macedonia. The Roma population consists of 77 healthy unrelated individuals, residents of different geographical regions of the Republic of Macedonia (Skopje, Gostivar, and Kochani). Blood samples were collected after obtaining written consent. DNA was isolated from peripheral blood and 22...

lentil (lens culinaris. medik) is one of the major cool season pulse crops which could suffer damage under severe cold. some physiological parameters such as electrolyte leakage (el) have been used to assess the response of crops to cold conditions. in order to evaluate the possibility of using the el index for assessing the freezing tolerance of lentil genotypes, a factorial experiment carried...

safflower (carthamus tinctorius l.) is one of the oilseed plants that can influence strongly on oilseed security because it is extremely compatible with environmental conditions. safflower is cultivated as second cultivation after combining cereals in isfahan. nowadays many attempts are performed to improve safflower oil quality to use in edible oil industry. in this study, quantity and quality...

Mahalanobis’ D-square (D2) statistics was applied to assess diversity in the 9 genotypes collectedof semi-arid region of India (7 genotypes from Gujarat and Rajasthan for normal toxic and two fromOrissa csmcri’s plantation of non toxic nature. These genotypes were grouped into five. Cluster I andIII had two genotypes, cluster II had three genotypes and cluster VI and V contributed as solitaryge...

To study the effect of phosphorylation on protein conformation, a fluorescence spectroscopic study was performed on phosphorylated enolase and histoneH1 proteins. The peak of fluorescence was 330 and 360 nm for each protein, respectively, when excited at 287 nm. The intensities of the fluorescence were measured during the phosphorylation reactions with the protein kinase A and p43v-abl, for ser...

A simple procedure for purification of ferredoxin from a green alga, Scenedesmus, is described. Crystalline ferredoxin had an absorption spectrum with maxima at 276, 330, 421, and 464 rnp and with minima at 255, 293, 390, and 445 rnp. The ratios of the optical densities at 421 and 330 rnp to that at 276 rnp were 0.651 and 0.883, respectively. The substance was shown to be pure by disc electroph...

A simple procedure for purification of ferredoxin from a green alga, Scenedesmus, is described. Crystalline ferredoxin had an absorption spectrum with maxima at 276, 330, 421, and 464 rnp and with minima at 255, 293, 390, and 445 rnp. The ratios of the optical densities at 421 and 330 rnp to that at 276 rnp were 0.651 and 0.883, respectively. The substance was shown to be pure by disc electroph...