نتایج جستجو برای: reporter gene

تعداد نتایج: 1151125  

Journal: :Current Protocols in Microbiology 2013

Journal: :Proceedings of the National Academy of Sciences 2008

2003
Carolina Vollert Peter Uetz

The bait and prey fusions are co-expressed in yeast where a physical interaction between X and Y leads to the reconstitution of a functional transcription factor that binds to the promoter of a reporter gene (Fig.1). Consequently, transcription of the reporter gene is switched on. A protein-protein interaction is therefore translated into reporter gene activity which can be detected or measured...

Journal: :Mutagenesis 2013
Derrik M Leach Natalie J Zacal Andrew J Rainbow

Previously, we have reported the use of a recombinant adenovirus (Ad)-based host cell reactivation (HCR) assay to examine nucleotide excision repair (NER) of UVC-induced DNA lesions in several mammalian cell types. The recombinant non-replicating Ad expresses the Escherichia coli β-galactosidase (β-gal) reporter gene under control of the cytomegalovirus immediate-early enhancer region. We have ...

Journal: :Chemistry & biology 2015
Liwei Lang Han-Fei Ding Xiaoguang Chen Shi-Yong Sun Gang Liu Chunhong Yan

Although transgene-based reporter gene assays have been used to discover small molecules targeting expression of cancer-driving genes, the success is limited due to the fact that reporter gene expression regulated by incomplete cis-acting elements and foreign epigenetic environments does not faithfully reproduce chemical responses of endogenous genes. Here, we present an internal ribosome entry...

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