Osteoporosis is one of the common degenerative skeletal diseases whose essential mechanism imbalance between bone resorption and formation. Currently, close coordination signaling pathways at genetic level during development a hot topic osteoporosis research. The present study focused on whether pterostilbene protects against hydrogen peroxide (H2O2)-induced osteoblastic cell apoptosis, oxidati...

objective(s): oxidative stress has been established as a key cause of alcohol-induced hepatotoxicity. licochalcone b, an extract of licorice root, has shown antioxidative properties. this study was to investigate the effects and mechanisms of licochalcone b in ethanol-induced hepatic injury in an in vitro study. materials and methods: an in vitro model of ethanol-induced cytotoxicity in brl cel...

Objective(s):Pharmacological studies showed that the extracts of Jin Yin Hua and its active constituents have lipid lowering, antipyretic, hepatoprotective, cytoprotective, antimicrobial, antibiotic, antioxidative, antiviral, and anti-inflammatory effects. The purpose of the present study was to investigate the protective effects of caffeoylquinic acids (CQAs) from Jin Yin Hua against hydrogen ...

LGK974 is a potent Wingless-related integration site inhibitor at Phase II of clinical trial. However, little known about its role and the underlying mechanisms in human hepatocellular carcinoma. 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide colony formation assays were used to determine cytotoxicity on liver cancer cell lines. Flow cytometry assay was performed examine apoptosis...

Abstract Background Secreted frizzled-related protein 5 (SFRP5) is an endogenous inhibitor of Wnt5a (wingless-type family member 5a), which has been implicated in anti-inflammatory response. In this study, we aimed to investigate whether SFRP5 could protect chondrocytes against LPS-induced inflammation and apoptosis. Methods ATDC5 cells that overexpressed with or not were challenged LPS observe...

In this issue of Cancer Discovery, Bellail and colleagues unravel how overexpression of the ubiquitin-modifying enzyme A20 results in TNF-related apoptosis-inducing ligand (TRAIL) resistance in glioblastoma. After TRAIL receptor stimulation, A20 mediates the polyubiquitination of RIP1 at the TRAIL receptor tail, resulting in the interaction of the polyubiquin chain to procaspase-8 that is recru...

When cells are exposed to death ligands such as TRAIL, a fraction undergoes apoptosis and a fraction survives; if surviving cells are re-exposed to TRAIL, fractional killing is once again observed. Therapeutic antibodies directed against TRAIL receptors also cause fractional killing, even at saturating concentrations, limiting their effectiveness. Fractional killing arises from cell-to-cell flu...

background: toxic effects of anti-cancer and other drugs on the normal tissues could be reduced by the herbal plants and their fractions. this study investigated the protective effect of thymoquinone (tq) as a fraction of nigella sativa on methotrexate (mtx)- induced germ cell apoptosis in male mice. materials and methods: in this experimental study, thirty male balb/c mice were divided randoml...