نتایج جستجو برای: recombinant hepta epitope peptide

تعداد نتایج: 276060  

Journal: :Journal of immunology 2002
E John Wherry Michael J McElhaugh Laurence C Eisenlohr

The magnitude of a virus-specific memory CTL population can dramatically influence the outcome of secondary infections, yet little is known about the determinants of memory size. We investigated the impact of epitope levels on CTL memory generation by using a recombinant vaccinia virus system that allows for a broad range of epitope expression with the same infectious dose of virus. The size of...

2011
Sumathy Velumani Hui-Ting Ho Fang He Syed Musthaq Mookkan Prabakaran Jimmy Kwang

BACKGROUND Active serologic surveillance of H5N1 highly pathogenic avian influenza (HPAI) virus in humans and poultry is critical to control this disease. However, the need for a robust, sensitive and specific serologic test for the rapid detection of antibodies to H5N1 viruses has not been met. METHODOLOGY/PRINCIPAL FINDINGS Previously, we reported a universal epitope (CNTKCQTP) in H5 hemagg...

Journal: :Journal of immunology 2010
Xiang-Yang Wang Xiaolei Sun Xing Chen John Facciponte Elizabeth A Repasky John Kane John R Subjeck

Our previous studies have demonstrated that the natural chaperone complexes of full-length tumor protein Ags (e.g., gp100) and large stress proteins (e.g., hsp110 and grp170) with exceptional Ag-holding capabilities augment potent tumor protective immunity. In this study, we assess the peptide-interacting property of these large chaperones and, for the first time, compare the immunogenicity of ...

Journal: :Biomedical journal 2021

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has caused a pandemic involving so far more than 22 million infections and 776,157 deaths. Effective vaccines are urgently needed to prevent SARS-CoV-2 infections. No have yet been approved for licensure by regulatory agencies. Even though host immune responses beginning be unravelled, effective clearance of virus will depend on both ...

2016
Iryna Dekhtiarenko Robert B Ratts Renata Blatnik Lian N Lee Sonja Fischer Lisa Borkner Jennifer D Oduro Thomas F Marandu Stephanie Hoppe Zsolt Ruzsics Julia K Sonnemann Mandana Mansouri Christine Meyer Niels A W Lemmermann Rafaela Holtappels Ramon Arens Paul Klenerman Klaus Früh Matthias J Reddehase Angelika B Riemer Luka Cicin-Sain

Cytomegalovirus (CMV) elicits long-term T-cell immunity of unparalleled strength, which has allowed the development of highly protective CMV-based vaccine vectors. Counterintuitively, experimental vaccines encoding a single MHC-I restricted epitope offered better immune protection than those expressing entire proteins, including the same epitope. To clarify this conundrum, we generated recombin...

2015
Daniela Gallerano Portia Ndlovu Ian Makupe Margarete Focke-Tejkl Kerstin Fauland Eva Wollmann Elisabeth Puchhammer-Stöckl Walter Keller Elopy Sibanda Rudolf Valenta

A comprehensive set of recombinant proteins and peptides of the proteome of HIV-1 clade C was prepared and purified and used to measure IgG, IgG-subclass, IgA and IgM responses in HIV-infected patients from Sub-Saharan Africa, where clade C is predominant. As a comparison group, HIV-infected patients from Europe were tested. African and European patients showed an almost identical antibody reac...

2010

INTRODUCTION Osteogenic differentiation and mineralization of bone marrow stromal (BMS) cells depends on the cells’ interactions with bioactive peptides associated with the matrix proteins. Multiple bioactive peptides in the bone ECM, interact with progenitor BMS cells to initiate the cascade of chemotaxis, differentiation, and mineralization. The peptide KIPKASSVPTELSAISTLYL (hereafter referre...

Journal: :The Journal of clinical investigation 1992
L J McNeilage K Umapathysivam E Macmillan A Guidolin S Whittingham T Gordon

High-titer IgG autoantibodies to the La/SS-B ribonucleoprotein (RNP) are a hallmark of patients with primary Sjogren's syndrome. Anti-La/SS-B-positive human sera bind to multiple epitopes on recombinant La/SS-B, although the initial response is against an immunodominant epitope within the first 107 NH2-terminal amino acids (aa). Sequence analysis has identified a striking homology between aa 88...

2014
Minglong Liu Xingui Tian Xiao Li Zhichao Zhou Chenyang Li Rong Zhou

The generation of monoclonal antibodies (MAbs) by epitope-based immunization is difficult because the immunogenicity of simple peptides is poor and T cells must be potently stimulated and immunological memory elicited. A strategy in which antigen is incorporated into the adenoviral capsid protein has been used previously to develop antibody responses against several vaccine targets and may offe...

2016
Wan-Xiang Xu Jian Wang Hai-Ping Tang Ya-Ping He Qian-Xi Zhu Satish K. Gupta Shao-Hua Gu Qiang Huang Chao-Neng Ji Ling-Feng Liu Gui-Ling Li Cong-Jian Xu Yi Xie

To enable rational multi-epitope vaccine and diagnostic antigen design, it is imperative to delineate complete IgG-epitome of the protein. Here, we describe results of IgG-epitome decoding of three proteins from high-risk (HR-) oncogenic human papillomavirus type 58 (HPV58). To reveal their entire epitomes, employing peptide biosynthetic approach, 30 precise linear B-cell epitopes (BCEs) were m...

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