Proline dehydrogenase (ProDH; 1.5.99.8) belongs to superfamily of amino acid dehydrogenase, which plays a significant role in the metabolic pathway from proline to glutamate. The goal of this research was gene cloning and characterization of ProDH enzyme from Pseudomonas fluorescens pf-5 strain. The gene encoding ProDH was isolated by means of PCR amplification and cloned in an IPTG inducible T...

D-amino acid oxidase from Trigonopsis variabilis (TvDAAO) was overproduced in Escherichia coli cells, and properties of the recombinant enzyme were studied. Single point mutants of the enzyme with 2.4-fold higher thermal stability or changed spectra of substrate specificity compared with wild-type enzyme were prepared. It was shown that mutant TvDAAO has higher catalytic efficiency in cephalosp...

objective: organophosphorus hydrolase (oph) is a homodimeric enzyme that can hydrolyze phosphoester bonds and reduce the toxicity of organophosphorus compounds. this makes oph a suitable element for the biodegradation of these compounds. methods: we successfully cloned the oph gene from pseudomonas diminuta, after optimization for pichia pastoris, into a yeast expression vector (ppiczαb). afte...

In this study, Rhizomucor miehei ?-1,3-1,4-glucanase gene was expressed under the regulation of AOX1 promoter in Pichia pastoris expression system, and clone providing highest production level determined. The codon-optimized ligated into vector pPICZ?A transferred competent P. KM71H cells. Thirty transformants were selected from plates containing different concentrations zeocin cultured test tu...