The major role of RecA is thought to be in helping repair and restart stalled replication forks. During exponential growth, Bacillus subtilis recA cells exhibited few microscopically observable nucleoid defects. However, the efficiency of plating was about 12% of that of the parent strain. A substantial and additive defect in viability was also seen for addB and recF mutants, suggesting a role ...

1.1 Recombinational repair Homologous recombination (HR) is essential for genetic diversity and genome stability. The conserved RecA-like recombinases promote pairing and consequent exchange of fragments between two homologous DNA molecules during conjugation in bacteria and meiotic recombination in eukaryotes. HR is a main DNA repair pathway particularly important in case of large-scale DNA da...