The polymerase chain reaction (PCR) has been widely used for diagnosis of infectious diseases of domestic animals. Rapid detection of respiratory pathogens of cattle is useful for making therapeutic decisions. Therefore, we developed a new genetic-based method called droplet-real-time PCR, which can detect bovine respiratory syncytial virus (BRSV) within 10 min. Our droplet-real-time PCR marked...

It is currently unknown if the use of a real-time polymerase chain reaction (PCR) adds value to the diagnosis and follow-up prognosis of patients affected by leishmaniasis. We performed a study using a real-time PCR directed against the alpha-polymerase gene and a semiquantitative PCR that target the SSU ribosomal RNA (rRNA) gene as control for the diagnosis and quantification of parasites in p...

Background and Objective: Sexually infections transmitted by bacteria are one of thetherapeutic and social problemsworldwide. The Real-time PCR assay is one of the most sensitive diagnostic and screening methods for these infections. The purpose of this study wassimultaneous detection of Chlamydia trachomatis and Mycoplasma genitaliumusing the TaqMan duplex real-time polymerase chain reaction. ...

canine parvovirus type 2 (cpv-2) is one of the most common viruses responsible for acute hemorrhagic enteritis in dogs. a rapid and accurate diagnosis of cpv-2 infection is especially important in kennels in order to isolate infected dogs. the aim of the present study was to compare two laboratory tests i.e., polymerase change reaction (pcr) and immunochromatography assay (ica) most commonly us...

BACKGROUND Recently, Stein et al. identified the metastasis-associated in colon cancer 1 (MACC1) gene by genome-wide search for differentially expressed genes in human colon cancer tissues and metastases. PATIENTS AND METHODS We analyzed MACC1 expression levels in 52 colorectal cancer samples using quantitative real-time polymerase chain reaction (QRT-PCR). RESULTS We found that MACC1 expre...

We analyzed minimal residual disease (MRD) by consensus polymerase chain reaction (PCR), quantitative PCR (qPCR), and flow cytometry in 40 patients with chronic lymphocytic leukemia (CLL) who underwent stem cell transplantation; 97.4%, 89%, and 100% of the patients could be studied by consensus PCR, qPCR, and flow cytometry, respectively. Overall, 164 of 248 samples were negative for MRD by con...

In article number 2100430 by Sung Gap Im, Kyoung G. Lee, and co-workers, an “all-in-one tube” is devised coating poly-(2-dimethylaminomethyl styrene) (pDMAMS) on a polymerase chain reaction (PCR) tube via initiated chemical vapor deposition (iCVD) to extract, amplify, detect pathogens within single tube. The all-in-one can capture the DNA of charge interaction directly perform real-time PCR, re...

Background: Sinonasal symptoms were usually reported to appear initially, yielding the important for early detection of coronavirus disease 2019 (COVID-19). This study was conducted retrospectively investigate detailed sinonasal manifestations and dynamic profile real-time reverse transcription polymerase chain reaction (RT-PCR) results severe acute respiratory syndrome 2 (SARS-CoV-2) in COVID-...