This study examined the potential utility of DNA sequence analysis to discriminate and identify plant material in forensic investigations. DNA was extracted from plant leaf fragments of 11 species stored for 5 to 22 years after collection. The trnH-psbA intergenic spacer and 316 bp of the rbcL gene were successfully amplified and sequenced for all fragments except for the trnH-psbA spacer of on...

We developed PCR primers against highly conserved regions of the rRNA operon located within the inverted repeat of the chloroplast genome and used these to amplify the region spanning from the 3' terminus of the 23S rRNA gene to the 5' terminus of the 5S rRNA gene. The sequence of this roughly 500-bp region, which includes the 4.5S rRNA gene and two chloroplast intergenic transcribed spacer reg...

Rice is the most important crop in the world as the staple food for over half of the population. The wild species of Oryza represent an enormous gene pool for genetic improvement of rice cultivars. Accurate and rapid identification of these species is critical for effective utilization of the wild rice germplasm. In this study, we developed valuable chloroplast molecular markers by comparing th...

The rbcL (ribulose-1,5-biphosphate carboxylase oxygenase) gene plays a crucial role in carbon fixation. Previous studies shed light on its evolutionary relationship among different Phyla. Here, authors have done a comparative study of rbcL genes among proteobacteria, archaea, cyanobacteria and plants based on their compositional variations (GC%, amino acid frequency, codon usage, etc.). In addi...

DNA barcoding of United Arab Emirates (UAE) native plants is of high practical and scientific value as the plants adapt to very harsh environmental conditions that challenge their identification. Fifty-one plant species belonged to 22 families, 2 monocots, and 20 eudicots; a maximum number of species being legumes and grasses were collected. To authenticate the morphological identification of t...

Abstract Pentatricopeptide repeat (PPR) proteins are helical repeat-proteins that bind RNA in a modular fashion with sequence-specificity can be manipulated by the use of an amino acid code. As such, PPR repeats promising scaffolds for design binding synthetic biology applications. However, vivo functional capabilities artificial built from consensus motifs just starting to explored. Here, we r...