We identify a novel activity of the RarA (also MgsA) protein of Escherichia coli, demonstrating that this protein functions at DNA ends to generate flaps. A AAA+ ATPase in the clamp loader clade, RarA protein is part of a highly conserved family of DNA metabolism proteins. We demonstrate that RarA binds to double-stranded DNA in its ATP-bound state and single-stranded DNA in its apo state. RarA...

Signaling from the human hematopoietic stem cell (HSC) niche formed by osteoblastic cells regulates hematopoiesis. We previously found that retinoic acid receptor alpha (RARa), a transcription factor activated by retinoic acid (RA), mediates both granulocytic and osteoblastic differentiation. This effect depends on decreased phosphorylation of serine 77 of RARa (RARaS77) by the cyclin-dependent...

Acute promyelocytic leukemia is a myeloid disorder that is characterized by the specific t(15;17) variant in ~98% of cases. The typical hypergranular and microgranular or hypogranular types exist, and are frequently associated with disseminated intravascular coagulopathy. Rare cases of promyelocytic leukemia-retinoic acid receptor α (PML-RARA) fusion without the reciprocal RARA-PML have been re...

Background: The secondary genetic changes other than the promyelocytic leukemia-retinoic acid receptor (PML-RARA) fusion gene may contribute to the acute promyelocytic leukemogenesis. Chromosomal alterations and mutation of FLT3 (FMS-like tyrosine kinase 3) tyrosine kinase receptor are the frequent genetic alterations in acute myeloid leukemia. However, the prognostic significance of FLT3 mutat...

introduction: the secondary genetic changes other than the pml-rara fusion gene may contribute to the acute promyelocytic leukemogenesis. chromosomal alterations and mutation of flt3 tyrosine kinase receptor are the frequent genetic alterations in acute myeloid leukemia (aml). however, the prognostic significance of flt3 mutations in acute promyelocytic leukemia (apl) is not firmly established....

Let G be a simple graph and A(G) its adjacency matrix. Based on some results of Rara (H. M. Rara, Discr. Math. 151 (1996) 213–219), we show that the determinant of A(G) of a plane graph G which has the property that every face-boundary is a cycle of size divisible by 4, equals −1, 0 or 1, provided the inner dual graph of G is a tree. As applications, we compute the algebraic structure count of ...

BACKGROUND AND OBJECTIVES In this study, we tested whether transgenic murine acute promyelocytic leukemia (APL) cells can be recognized and cleared by adaptive immune responses and/or vaccination strategies. DESIGN AND METHODS Immunocompetent and SCID mice were examined for their ability to survive a challenge of APL cells. We also vaccinated immunocompetent mice with DNA vaccines encoding va...

A unique mRNA produced by the t(15;17)(q22-24;qll21) translocation in the leukemic cells of acute promyelocytic leukemia patients encodes a chimeric protein, PML/ RARa, which is formed by the fusion of the retinoic acid receptor a (RARa) and the promyelocytic locus gene (PML). This translocation is often the only visible karyotypic aberration present which is detected in almost 100% of acute pr...

Acute Promyelocytic Leukemia (APL) is one of the most curable leukemia which shows great sensitivity to all-trans retinoic acid (ATRA) although a small number of the patients present poor prognosis and short survival. Isochromosome 17 in APL which usually bears an additional copy of RARA/PML fusion gene is considered to be a negative factor on its prognosis. Cryptic t(15;17) on i(17q) leads to ...