ABSTRACT A random amplified polymorphic DNA (RAPD) marker directly linked (0.0 cM) with a resistance gene was identified in a snap bean recombinant inbred population (Moncayo x Primo) consisting of 94 F(5:7) recombinant inbred lines that had uniform segregation for disease reaction to Beet curly top virus (BCTV) across three field locations. Resistance was conditioned by a single dominant allel...

Karnal bunt of wheat, caused by Tilletia indica Mitra is an important disease prevalent in several countries. The pathogen due to its heterothallic nature shows high variability. In the present study, genetic relationships among ten isolates of T.indica collected from different locations of India and 15 monosporidial lines raised from these isolates were investigated by using 34 RAPD and 28 ISS...

The combined use of the arbitrarily primed polymerase chain reaction [AP-PCR, also known as random amplification of polymorphic DNA (RAPD}] and isozyme mapping resulted in the production of 87 potential marker loci, enabling an overall expansion within the genetic map of the fish genus Xiphophorus. Use of DNA sequencing-style acrylamide gels and carefully controlled conditions of amplification ...

Molecular characterization of eleven clones of C. winterianus collected from eleven districts of West Bengal was carried out with 10 RAPD and 9 ISSR primers. The RAPD primers developed 81 robust loci, which revealed 74.07% polymorphism and 61 ISSR markers generated 47.50% polymorphism. Out of the different anchored primer combinations, ISSR primers with AG and GA motifs produced clear and maxim...

ABSTRACT Collections of Puccinia triticina, the wheat leaf rust fungus, were obtained from Great Britain, Slovakia, Israel, Germany, Australia, Italy, Spain, Hungary, South Africa, Uruguay, New Zealand, Brazil, Pakistan, Nepal, and eastern and western Canada. All single-uredinial isolates derived from the collections were tested for virulence polymorphism on 22 Thatcher wheat lines that are nea...

The Solanum brevidens genome (2n = 2x = 24) was examined with randomly applied polymorphic DNA (RAPD) markers in a second backcross population derived from a S. brevidens + S. tuberosum somatic hybrid. RAPD markers cosegregated into 12 different S. brevidens synteny groups. Most synteny groups were nonrecombinant. However, nearly 40% of the S. brevidens synteny groups detected in this populatio...

Micropropagated plantlets derived from three different grape rootstock genotypes namely, Dogridge (Vitis champini), SO4 (V. berlandieri×V. rupestris) and ARI-H-144 (V. vinifera×V. labrusca) were subjected to randomly amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) analyses in order to evaluate their genetic stability and/or detect likely existing variations among in vi...

Kumar A, Verma N. 2012. A comparative phylogenetic analysis of medicinal plant Tribulus terrestris in Northwest India revealed by RAPD and ISSR markers. Biodiversitas 13: 107-113. Several DNA marker systems and associated techniques are available today for fingerprinting of plant varieties. A total of 5 RAPD and 8 ISSR primers were used. Amplification of genomic DNA of the 6 genotypes, using RA...

Rust, incited by Uromyces appendiculatus^ is a major disease in common bean {Phaseolus vulgaris L,). Plant growth habit is also an important trait. Indeterminate growth habit was first reported to be controlled by a single dominant gene (Fin) (Lamprecht, 1935). Molecular markers such as isozymes, RFLP, and RAPD, have been used to tag genes for many traits, particularly disease resistance in sev...