Background: Many techniques have been developed to increase DNA molecules into sperms called sperm mediated gene transfer (SMGT) and one of them is electroporation. Sperm mediated gene transfer is an ideal method for mass gene transfer in organisms with external fertilization. Gene transfer is applied to rainbow trout sperm and the effects of electroporation on sperms and rate of gene uptake by...

The Pacific salmonid species Oncorhynchus mykiss is separated into a migratory form (steelhead trout) and a non-migratory form (rainbow trout). A decrease in water temperature is likely a cue triggering downstream behavior in the migratory form, and testosterone inhibits onset of this behavior. To elucidate differences in sensitivity to water temperature decreases between the migratory and non-...

Homozygous and hybrid clonal lines of rainbow trout (Oncorhynchus mykiss) were confirmed to be isogenic using multilocus DNA fingerprinting. Homozygous clones were produced by androgenesis and gynogenesis using gametes from androgenetic male and female rainbow trout, respectively. Isogenic F1 hybrid lines were produced by crossing homozygous fish from different strains. One line of hybrid clone...

Rainbow trout, Oncorhynchus mykiss, vitellogenin (Vtg) was purified from plasma of E2-treated male by direct anion exchange chromatography and some of its biochemical characteristics were studied. Our results demonstrated that, under SDS-PAGE conditions, rainbow trout Vtg was composed of two molecular forms of 390 and 176 kDa representing, respectively, the dimeric form and the monomeric from o...

Exposure to environmental estrogenic alkyl phenolic chemicals in rainbow trout resulted in a decreased fertility and egg production in females, reduction of gonadal size (Jobling et al., 1996; Ashfield et al., 1998), reduced sperm production and demasculinisation of genetic male Japanese medaka, intersex gonads (Nimrod and Benson, 1998; Gimeno et al., 1998) etc. Another frequently observed effe...

The acute toxicity of cadmium, copper, and zinc to white sturgeon (Acipenser transmontanus) and rainbow trout (Oncorhynchus mykiss) were determined for 7 developmental life stages in flow-through water-only exposures. Metal toxicity varied by species and by life stage. Rainbow trout were more sensitive to cadmium than white sturgeon across all life stages, with median effect concentrations (har...

been reported in fish, all of them in trout (924,34, 47). In 1955 a high incidence of hepatic tumors was found in rainbow trout in Italian hatcheries (9). Interest in these neoplasms was renewed recently when hepatic tumors were discovered in large numbers of hatchery-reared rainbow trout in the United States (928,35, 592,61, 64), Italy (920), and France (3, 30). The main varieties of tumors th...

distribution of infectious hematopoietic necrosis (ihn) was studied in five major provinces producing rainbow trout(oncorhynchus mykiss)fry in iran by indirect fluorescence antibody ifatand nested-rt-pcr techniques. also the effect of time duration was examined on some positive samples after 8 months post sampling. samples of kidney, liver and spleen of rainbow trout fries collected from 27 tro...

Estrogen receptor-mediated induction of zona radiata (ZR) and vitellogenin (VTG) mRNA and protein in rainbow trout (Oncorhynchus mykiss) was compared to assess their utility as biomarkers for exposure to estrogenic compounds. Partial sequences of rainbow trout ZR and beta-actin were cloned by reverse transcriptase polymerase chain reaction (RT-PCR) using degenerate primers based on conserved re...

The genome sequence of Flavobacterium psychrophilum strain CSF259-93, isolated from rainbow trout (Oncorhynchus mykiss), consists of a single circular genome of 2,900,735 bp and 2,701 predicted open reading frames (ORFs). Strain CSF259-93 has been used to select a line of rainbow trout with increased genetic resistance against bacterial cold water disease.