On the basis of Eu(III)-4,7-bis(chlorosulfophenyl)-1,10-phenanthroline-2,9-di-carboxylic acid chelate (BCPDA) that was synthesized and characterized for time-resolved fluoroimmunoassay (TRFIA), Donkey anti-hepatitis B surface (anti-HBs) was labeled with BCPDA-Eu3+. Coomassie Brilliant Blue was used to determine the protein concentration and radio immunoassay (RIA) for detecting the biological a...

The objective of this study was to determine endothelin (ET-1) concentrations in monochorionic twin fetuses with and without twin-twin transfusion syndrome (TTTS). Fourteen monochorionic twin pregnancies complicated by TTTS and six without TTTS were studied. Matched maternal and fetal blood samples were obtained both in utero and at birth. Amniotic fluid samples were also collected from twin pa...

Sera from 5,244 blood donations collected between 1979 and 1987 were screened for antibody to HTLV-I with an enzyme immunoassay (EIA) whose result was confirmed with a two-step procedure including Western blot (WB) and radio immunoprecipitation. Of 20 repeatedly reactive samples, two were confirmed positive for HTLV-I infection. These blood units were transfused to patients undergoing cardiac s...

background and objectives: there are different types of vascular access. we compared the patency rate for six months in ulnar-basilic fistula and radio-cephalic fistula for vascular access in hemodialysis patients.   methods: the study was performed on 60 patients of 50.6±13.5 years of age with end-stage renal failure undergoing hemodialysis from 2012-2014 in imam ali hospital in zahedan. patie...

in this paper, a new propagation model based on utd for multiple diffraction paths in cellular mobile radio communications in urban environments is proposed. moreover, the most rigorous novel utd-based expressions for multiple diffractions by buildings and excess path losses are d - rived and analyzed. for this purpose, building rows are supposed to have rectangular cross-sections with the same...

objective: the prevalence of respiratory allergies, especially those induced by fungi such as alternaria alternata, has dramatically increased over the past decade. this increase has caused major health problems worldwide. this study aimed to investigate the role of a. alternata in the etiology of allergic asthma, by using the skin prick test and assessment of ige specific to the fungus in the ...

Background and Objectives: There are different types of vascular access. We compared the patency rate for six months in ulnar-basilic fistula and Radio-Cephalic fistula for vascular access in hemodialysis patients.   Methods: The study was performed on 60 patients of 50.6±13.5 years of age with end-stage renal failure undergoing hemodialysis from 2012-2014 in Imam Ali hospital in Zahedan. Pat...

A new Enzyme Linked Immuno Sorbent Assay (ELISA) kit for the determination of serum ferritin has been compared with another ferritin kit based on the Immuno Radio-Metric Assay (IRMA) approach, both assays containing similar antibodies. Based on these studies, we found the within-run precision of the ELISA (and IRMA) to have coefficients of variation of 4-10% and 2-6% respectively, over a concen...

The radio-immunological technique for assaying endogenous plasma insulin in man recently described by Yalow and Berson (1, 2) promises to be of considerable value in the investigation of insulin metabolism. Other immunological methods that have been reported are not so sensitive as this method, since they fail to detect insulin in fasting normal human plasma (3). In contrast to the bioassay met...

A total of 138 serum samples submitted for toxoplasma serology have been examined by enzyme immunoassay using kits produced by Labsystems Oy for the detection of specific antibodies of the IgG and IgM class. Results were compared with the dye test, an indirect haemagglutination test, and an indirect immunofluorescence test for specific IgM. The enzyme immunoassay was less sensitive than the dye...