Excretion of alpha-keto acids by clinical isolates and laboratory strains of Salmonella typhimurium was determined by high-performance liquid chromatography analysis of culture supernatants. The levels of excretion increased markedly with increasing iron stress imposed by the presence of alpha,alpha'-dipyridyl or conalbumin in the medium. The major product was pyruvic acid, but significant conc...

Recent experiments,' carried out with the aid of labeled carbon, have shown beyond any doubt that CO2 enters into the metabolism of a large variety of organisms in the absence of light. As a consequence it has been suggested that CO2 assimilation may be a process of wide-spread occurrence in living systems. The metabolic processes of living organisms are for the most part enzyme catalyzed. The ...

In 1932, Bergmann and Schleich (1) observed that glycerol extracts of swine kidney and pancreas possess the capacity of hydrolyzing glycyldehydrophenylalanine (glycyl-cr-aminophenylacrylic acid) to glycine, ammonia, and phenylpyruvic acid. To the enzyme system responsible, whose distinction from dipeptidase, carboxypeptidase, and aminopeptidase was demonstrated, they gave the designation of deh...

Best, Audrey N. (University of Georgia, Athens), and W. J. Payne. Preliminary enzymatic events in asparagine-dependent denitrification by Pseudomonas perfectomarinus. J. Bacteriol. 89:1051-1054. 1965.-The initial events leading to denitrification by Pseudomonas perfectomarinus grown anaerobically in the presence of NO(3) (-) were investigated. Conversion of asparagine to malic acid was demonstr...

Attempts to elucidate carbohydrate metabolism in muscular diseases have been made by a relatively large number of authors but the results obtained, often contradictory, seem to be difficult to classify in a cycle of metabolic disorders. Nevertheless disturbances of carbohydrate metabolism appear to play an important role in the pathogenesis of muscular diseases. In view of the key position of p...

A compound derived from pyruvic acid and homocysteine has been isolated from reaction mixtures containing serinel*C, homocysteine, and serine dehydratase preparations. The formation of this product interferes with commonly used assays of serine dehydratase, which are based on the serine, pyruvic acid, or homocysteine content of incubation mixtures. Improved assay procedures have permitted the i...

1N11 ANY clinical and experimental problems concerning anaerobic metabolism and the accumulation of an oxygen debt have recently been studied, using Huckabee’s concept of “excess lactate” (1). Colonmetric methods have most often been used to measure pyruvic and lactic acid in blood (2, 3). The estimation of these two metabolites by an enzymatic analysis has advantages-e.g., specificity and ease...

glyoxal (or like substance) in human milk, while he has since been doubting if pyruvic acid does ever play any all-important role in the disease.3) But some pediatricians want to ignore his theory, as, since the Embden-Meyerhof scheme of carbohydrate metabolism became known, pyruvic acid has come into favor, while methyl glyoxal seems to have lost in favor. I am inclined to say that our Clinic ...

We examined the lactic and pyruvic acid levels in the plasma of 14 patients with migraine, 17 patients with tension-type headache, and 12 normal controls. The lactic and pyruvic acid levels in the plasma of the migraine patients were significantly higher than those of the normal controls (9.6 +/- 5.0 mg/dL and 0.51 +/- 0.30 mg/dL versus 3.3 +/- 1.9 mg/dL and 0.26 +/- 0.20 mg/dL, respectively). ...

L-Valine solutions in water and phosphate buffer were treated with nonthermal plasma generated by using a dielectric barrier discharge (DBD) device and the products generated after plasma treatments were characterized by (1)H NMR and GC-MS. Our results demonstrate that L-valine is decomposed to acetone, formic acid, acetic acid, threo-methylaspartic acid, erythro-methlyaspartic acid, and pyruvi...