A new direct method has been developed to determine protein-drug binding based on hollow fiber membrane solvent microextraction. Hollow-fiber membrane solvent microextraction coupled with high-performance chromatography with UV detection was employed to evaluate the binding characteristics of drugs to bovine serum albumin (BSA) and blood serum. It was found that the BSA and matrix in the blood ...

The aptitude of a hollow-fiber membrane reactor to determine lipase kinetics was investigated using the hydrolysis of triacetin catalyzed by lipase from Canadida cylindracea as a model system. The binding of the lipase to the membrane appears not to be very specific (surface adsorption), and probably its conformation is hardly altered by immobilization, resulting in an activity comparable to th...

Polyvinylidene fluoride (PVDF) ultrafiltration (UF) membrane combined with polyvinylidene fluoride-graft-2-hydroxyethyl acrylate (PVDF-g-PHEA) was fabricated via non-solvent induced phase separation (NIPS). In this study, PVDF-g-PHEA was synthesized via atom transfer radical polymerization (ATRP) method, and then synthesized graft copolymer was characterized using Fourier transform infrared spe...

Plasma-etching is a powerful tool in the determination of the resistance of the sublayer of skinned asymmetric hollow fiber membranes of poly (2,6-dimethyl-1,4-phenylene oxide). Using a resistance model and data provided by etched hollow PPO fiber membranes it is possible to predict the ultimate selectivity of the total membrane for helium over nitrogen and oxygen over nitrogen. From this model...

in this research, process asymmetric polyetherimide hollow fiber membranes using ethanol (0, 2 and 4 wt%) as non-solvent additive in the polymer dope via phase inversion method were fabricated. aqueous solution of 1-methyl-2-pyrrolidine (nmp) (90%) was applied as a bore fluid to avoid inner skin layer formation and water was used as the external coagulant. the morphology of fabricated membranes...

Two opposite design strategies for ultrafiltration/microfiltration filters, (1) reduction of concentration polarization and particle deposition to increase permeate velocity and (2) utilization of particle deposition on membrane surface to produce an additional (to permeate) volume of clarified water, are analyzed. It is shown that the first strategy is always associated with additional expendi...