background toxoplasma gondii is an obligatory intracellular protozoan parasite, which infects human beings. since the current antigens used for diagnosis or vaccination are contaminated with non parasitic material in which the parasite is grown, it is tried to produce recombinant antigens to design vaccines against toxoplasmosis, or make diagnostic kits. choosing the type of antigen to produce ...

background: streptokinase is a potent activator of plasminogen to plasmin, the enzyme that can solubilize the fibrin network in blood clots. streptokinase is currently used in clinical medicine as a thrombolytic agent. it is naturally secreted by β-hemolytic streptococci. methods: to reach an efficient method of purification, an immunoaffinity chromatography method was developed that could puri...

Structural studies of G protein-coupled receptors (GPCRs) gave insights into molecular mechanisms of their action and contributed significantly to molecular pharmacology. This is primarily due to technical advances in protein engineering, production and crystallization of these important receptor targets. On the other hand, NMR spectroscopy of GPCRs, which can provide information about their dy...

We demonstrate coherent control of bond making, a milestone on the way to coherent control of photoinduced bimolecular chemical reactions. In strong-field multiphoton femtosecond photoassociation experiments, we find the yield of detected magnesium dimer molecules to be enhanced for positively chirped pulses and suppressed for negatively chirped pulses. Our ab initio model shows that control is...

The intrinsic defects in reduced graphene oxide (rGO) formed during reduction processes can act as nanopores, making rGO a promising ultrathin-film membrane candidate for separations. To assess the potential of rGO for such applications, molecular dynamics techniques are employed to understand the defect formation in rGO and their separation performance in water desalination and natural gas pur...

This document contains the following supplementary figures and information further describing our bidirectional ITP-based technique for simultaneous purification and fractionation of nucleic acids and proteins from complex biological samples: • Figure S-1: Images of on-chip DNA ITP zones and protein ITP zones • Figure S-2: Experimental setup and procedure • Figure S-3: Simulation and visualizat...

background: botulinum neurotoxin (bont) complexes consist of neurotoxin and neurotoxin-associated proteins. hemagglutinin-33 (ha-33) is a member of bont type a (bont/a) complex. considering the protective role of ha-33 in preservation of bont/a in gastrointestinal harsh conditions and also its adjuvant role, recombinant production of this protein is favorable. thus in this study, ha-33 was expr...

background and objective: use of elastin-like proteins (elps) provides high-performance protein purification without need for chromatography. in line with cost reduction and facilitation of recombinant proteins purification, which represent a high percentage of production costs, in this project, we eliminated the need for proteases in the process of separation of recombinant proteins from elp b...

Once a protein purification scheme has been developed, the purification, characterization, and use/structure of a target protein are usually published. It is highly desirable to present the major steps in the purification and the corresponding features of the protein at each step summarized in the form of a purification summary table. In considering whether to repeat a published protein purific...

The toxicity and corrosion potential of hydrogen sulfide in raw biogas underlines the need for biogas purification. Several techniques available for removal of hydrogen sulfide from biogas are out of the reach for common end users due to lack of knowledge, higher running costs, and insufficient operational skills. The present experimental study aims to propagate hydrogen sulfide removal techniq...