1Unit of Recombinant Protein, Institut Pasteur de Montevideo, Montevideo, Uruguay; 2Department of Immunology, Institute for Hematologic Research, National Academy of Medicine, Buenos Aires, Argentina; 3Unit of Cellular Biology, Institut Pasteur de Montevideo, Montevideo, Uruguay; 4Service of Hematology and Bone Marrow Transplantation, Hospital Maciel, Montevideo, Uruguay; 5Institut Pasteur de M...

Phospholipids and proteins are assumed to be components of cellular membranes. Both these substances have groups ionizable in aqueous media. We attempted to ascertain to what extent the behavior of cellular membranes is similar to that of nonbiological ion-exchange interfaces. One approach is the measurement of the dependence of the membrane potential in nerve upon the extracellular concentrati...

The reversibility and cation selectivity of the K(+)-Cl(-) cotransporter (KCC), which normally extrudes Cl(-) out of neurons, was investigated in dissociated lateral superior olive neurons of rats using the gramicidin perforated patch technique. Intracellular Cl(-) activity (alpha[Cl(-)](i)) was maintained well below electrochemical equilibrium as determined from the extracellular Cl(-) activit...

Microfluidics has emerged as a powerful laboratory toolbox for biologists, allowing manipulation and analysis of processes at a cellular and sub-cellular level, through utilization of microfabricated features at size-scales relevant to that of a single cell. In the majority of microfluidic devices, sample processing and analysis occur within closed microchannels, imposing restrictions on sample...

In chemical measurements (for instance in titrimetric analysis) it is very important to precisely measure amount of liquid, the measurement is performed with use of various measuring vessels. Presently laboratories use all kinds of glass measuring vessels like: beakers, flasks, etc., for measurement of bigger volumes of liquid, and in case of small volumes glass pipettes. Glass pipettes have be...