Pseudomonas strains have shown promising results in biological control of late blight caused by Phytophthora infestans. However, the mechanism(s) and metabolites involved are in many cases poorly understood. Here, the role of the cyclic lipopeptide massetolide A of Pseudomonas fluorescens SS101 in biocontrol of tomato late blight was examined. Pseudomonas fluorescens SS101 was effective in prev...

Potato ranks fourth as the most important food crop in the world. The crop is known to suffer from many diseases among which late blight is more devastating. Therefore, culture filtrates of bio-control agents, (Trichoderma virens, Trichoderma viride, Paecilomyces lilacinus and Pseudomonas fluorescens) were tested for their effectiveness on the spore germination and infection in-vitro, as fungic...

Plasmid pHF360 was constructed from cloned rRNA genes (rDNA) of Pseudomonas aeruginosa and used as hybridization probe for the Pseudomonas fluorescens group. The probe was tested by dot and in situ colony hybridizations to chromosomal DNAs from a wide variety of organisms. pHF360 DNA hybridized exclusively to chromosomal DNAs from bacteria representing the P. fluorescens group and separated the...

Cellulase-producing bacteria were isolated from soil and identified as Pseudomonas fluorescens, Bacillus subtilIs, E. coli, and Serratia marcescens. Optimization of the fermentation medium for maximum cellulase production was carried out. The culture conditions like pH, temperature, carbon sources, and nitrogen sources were optimized. The optimum conditions found for cellulase production were 4...

The degree of barotolerance exhibited by Pseudomonas fluorescens and Pseudomonas bathycetes in vitro polyphenylalanine-synthesizing systems can be modified by altering the concentrations of specific ions in the reaction mixture. Hybrid-protein-synthesizing systems, utilizing all the possible S-100 supernatant fluid and ribosome combinations from Escherichia coli, P. fluorescens, and P. bathycet...

The lsp gene encoding prolipoprotein signal peptidase (signal peptidase II) is organized into an operon consisting of ileS and three open reading frames, designated genes x, orf149, and orf316 in both Escherichia coli and Enterobacter aerogenes. A plasmid, pBROC128, containing a 5.8-kb fragment of Pseudomonas fluorescens DNA was found to confer pseudomonic acid resistance on E. coli host cells ...

Pseudomonas fluorescens DSM 8569 represents a natural isolate of the rhizosphere of oilseed rape (Brassica napus) in Germany and possesses antagonistic potential toward the fungal pathogen Verticillium. We report here the draft genome sequence of strain DSM 8569, which comprises 5,914 protein-coding sequences.

Rhapidosomal rods released from strain 3~ of Pseudomonas JEuorescens appear to be polymerized sheaths of pyocin which had a structure like that of T-even phage tails. This conclusion was based on the following observations : ( I ) Morphologically the rods have the same structure and diameter as the contracted sheath of R-type pyocin. (2) They resemble the polysheath of T-even phages. (3) Antige...

Pseudomonas fluorescens ET76 was isolated from rice rhizosphere in northwestern Morocco. Its draft genome was estimated to be 6,681,652 bp with 5,789 coding sequences (CDSs). Genes encoding for type I to VI secretion systems, PvdQ, proteases, siderophores, hydrogen cyanide synthase, ACC-deaminase, among others, highlight its potential use in biological control of plant pathogens.

Twenty Pseudomonas fluorescens strains were isolated from rice growing soil samples and characterized. One of the P.fluorescens isolated and identified from the dual culture test. It was fermented for secondary metabolite in a small scale and extracted with ethyl acetate. The isolated metabolite tested against rice fungal pathogens. The structure of the compound was elucidated by high-resolutio...