The popular ornamental plant Petunia is also a valuable model in tissue culture. Cellular conversions during differentiation and regeneration have been investigated using various combinations of phytohormones; however, studies on genes for reprogramming toward desired identities limited. In this study, we isolated protoplasts cultured them the callus, rooting, or shooting stages, which were use...

The inhibitory allelopathic activities of leaves and leaf-origin suspension cultured cells Spiraea thunbergii, putative allelochemicals, cis- trans-cinnamic acid, were investigated by the protoplast co-culture method with digital image analysis (DIA-PP method) using lettuce as a recipient. optimal conditions 2,4-dichlorophenoxyacetic acid (2,4-D) benzyladenine (BA) for cell division S. thunberg...

Twenty-eight types of lysozyme-sensitive spores among seven Bacillus species representative of thermophiles, mesophiles, and psychrophiles were obtained spanning a 3,000-fold range in moist-heat resistance. The resistance within species was altered by demineralization of the native spores to protonated spores and remineralization of the protonated spores to calcified spores and by thermal adapt...

BACKGROUND Populus is a model woody plant and a promising feedstock for lignocellulosic biofuel production. However, its lengthy life cycle impedes rapid characterization of gene function. METHODOLOGY/PRINCIPAL FINDINGS We optimized a Populus leaf mesophyll protoplast isolation protocol and established a Populus protoplast transient expression system. We demonstrated that Populus protoplasts ...

Background: In Algeria, date palm is currently confronted to the Bayoud disease. Biotechnological tools such as protoplasts fusion can appear as an alternative to ensure rapid multiplication and improvement of this species. Objectives: Callogenesis induction in protoplasts isolated from embryogenic callus of three date palm cultivars. Materials and Methods: Some factors influencing the isolatio...

The formation of protoplast chains in suspensions of isolated pea (Pisum sativum cv Ran 1) mesophyll protoplasts induced by electric fields was studied. The chain formation induced by a sine-wave field (2 V, peak to peak; 500-0.1 kHz) was compared to that induced by an alternating pulse field (1 V, amplitude; 0.1-0.4 kHz). An increased number of dielectrophoretically paired protoplasts, formati...

Mass culture of algae for the production of biofuels is a developing technology designed to offset the depletion of fossil fuel reserves. However, large scale culture of algae in open ponds can be challenging because of incidences of infestation with algal parasites. Without knowledge of the identity of the specific parasite and how to control these pests, algal-based biofuel production will be...

Protoplast fractionation techniques were used to prepare subcellular fractions of three strains of Staphylococcus aureus for the analysis of alpha-hemolysin activity. More than 90% of the cellular hemolytic activity was localized in the periplasm, only a trace was detected in the cytoplasm, and none was found in either plasma membranes or mesosomes. This cellular portion constituted approximate...

The organization of actin microfilaments was studied by immunofluorescence isolated from sunflower hypocotyls and cultured in an agarose matrix. Remov wall completely disrupted the actin cytoskeleton, which became progressively into cortical microfilament arrays and actin cables during protoplast culture. protoplasts with Arg-Gly-Asp motif-containing peptides, to inhibit putative cell the agaro...