This first-attempt study used microtome-based methods to generate a thin cell layer culture for the micropropagation of Phal. Hwafeng Redjewel × New Cinderella. Protocorms were embedded in various agarose concentrations (8–12%, w/v) and dried from 1 8 h before sectioning with microtome. Optimal conditions slicing sections 100 300 μm achieved when protocorms at 10% (w/v) 4 under laminar flow, ha...

Synthetic seeds were prepared in Malaxis acuminata by encapsulating shoot tip derived protocorm-like bodies (PLBs; 0.2-0.5 cm) a hydrated gel capsule composed of calcium alginate matrix (sodium alginate; 3.5% and chloride; 75 mM) liquid Murashige Skoog, 1962 (MS) medium. varied physical features with the concentration gelling agent chloride. In vitro germination, optimum response was achieved M...