Genome editing technologies such as clustered regularly interspaced short palindromic repeats (CRISPR) systems have ushered in a new era of targeted DNA manipulation. The easy programmability of CRISPR using short oligonucleotides enables rapid synthesis of large-scale libraries for functional genetic screens. Here we present fundamental concepts and methods for pooled CRISPR screens and review...

This article presents the electric field distribution E and dielectric losses ?Pdiel. in insulation system of high-voltage cables. Such a consists inner outer semiconductor screens XLPE insulation. The aim this study was to compare values between objects research were cables 110 kV, 220 400 500 kV. geometrical dimensions cables, especially radii individual layers insulation, as well electrical ...

We address curved IR screens for multiwavelength systems. To first-order of the approximation, a curved screen may be viewed as composed of many local flat screens. On the other hand, the validity of such an approximation is not clear a priori. We provide experiments and simulations to show that such an approximation works well for cylindrically curved IR screens while monitoring their peak tra...

This paper presents a Heideggerian phenomenological analysis of screens. In a world and an epoch where screens pervade a great many aspects of human experience, we submit that phenomenology, much in a traditional methodological form, can provide an interesting and novel basis for our understanding of screens. We ground our analysis in the ontology of Martin Heidegger’s Being and Time [1927/1962...

Studies have suggested that flies play a linking role in the epidemiology of Campylobacter spp. in broiler chickens and that fly screens can reduce the prevalence of Campylobacter spp. We examined the year-round and long-term effects of fly screens in 10 broiler chicken houses (99 flocks) in Denmark. Prevalence of Campylobacter spp.-positive flocks was significantly reduced, from 41.4% during 2...

The aim of this study was to compare in vitro the protective and antioxidant properties lutein astaxanthin on human primary corneal epithelial cells (HCE-F). To purpose, HCE-F were irradiated with a blue-violet light lamp (415–420 nm) at different energies (20 80 J/cm2). Lutein (50 250 μM) added right before irradiation 50 J/cm2. Viability evaluated by CKK-8 assay while production reactive oxyg...

UNLABELLED Touch screens are popular nowadays as seen on public kiosks, industrial control panels and personal mobile devices. Numerical typing is one frequent task performed on touch screens, but this task on touch screen is subject to human errors and slow responses. This study aims to find innate differences of touch screens from standard physical keypads in the context of numerical typing b...