Garlic ( Allium sativum L) is one of genus sp which propagated vegetatively. In vegetative propagation, virus the major diseases. However, it has been approved that OYDV Onion Yellow Dwarf Virus ) can be eliminated through unconventional propagation techniques. The aim this research was to evaluate effect explant, variety and heat treatment on growth development explants dealing with eliminatio...

fars province is one of the natural habitats of pyrus species in iran. harvesting of fruits, deforestation, expansion of agriculture and overgrazing in recent years has threatened the wild pear open forests and imposed detrimental effects on their natural regeneration, therefore, investigation on propagation of wild pear species for afforestation is needed. this research was carried out to deve...

To know what a physio-chemical situation in the micro-propagating banana variety Basrai under saline conditions, an experiment was conducted. In vitro plantlets were developed by culturing meristematic stem tips (explant) for 3 weeks on organogenesis (8.0 μM BA; 10.0 μM IAA; 3.0 g l phytagel), 3 weeks on shoot induction (15.0 μM BA; 1.0 g l phytagel) and 4 weeks on shoot multiplication (10.0 μM...

A rapid in vitro propagation system leading to the formation of shoots, calli, roots, cell suspensions and plantlets was developed for the Andean medicinal plant Fabiana imbricata (Solanaceae). Massive propagation of shoots and roots was achieved by the temporary immersion system (TIS), morphogenesis and maintenance of cell suspensions by standard in vitro culture techniques. Oleanolic acid (OA...

Thymidine-phosphorylating capacity, mediated by thymidine kinase or a nucleoside phosphotransferase, is closely correlated with DNA synthesis and cell division in a wide variety of bacterial, animal, and plant systems (4, 5, 11, 14, 17, 22). In cells that are not undergoing DNA synthesis and cell division, these enzymes are usually low or undetectable. Conversely, systems undergoing rapid DNA s...

A non-destructive cultivation system was developed to study the dynamics of phosphorus (Pi) uptake by mycorrhizal and non-mycorrhizal maize plantlets. The system consisted of a plant container connected via silicon tubes to a glass bottle containing a nutrient solution supplemented with Pi. The nutrient solution is pumped with a peristaltic pump to the upper part of the container via the silico...

An efficient method was developed for plant regeneration and establishment from alginate encapsulated synthetic seeds of Rauvolfia serpentina. Synthetic seeds were produced using in vitro proliferated microshoots upon complexation of 3% sodium alginate prepared in Llyod and McCown woody plant medium (WPM) and 100 mM calcium chloride. Re-growth ability of encapsulated nodal segments was evaluate...

To unravel the functions of cell wall and vacuolar invertases in carrot, we used an antisense technique to generate transgenic carrot plants with reduced enzyme activity. Phenotypic alterations appeared at very early stages of development; indeed, the morphology of cotyledon-stage embryos was markedly changed. At the stage at which control plantlets had two to three leaves and one primary root,...