secreted cytokines of th1 (t-helper)/th2 cells play an important role in the pathogenesis of many diseases. th1 cells secrete predominantly ifn-γ and il-2 which regulate cell-mediated immunity against intracellular pathogens and tumors. in this study, expression of ifn-γ was studied using semiquantitative rt-pcr. in brief, lymphocytes of a healthy donor were stimulated with pha (1μg/106 cell/ml...

Environmental microbes oscillate between feast and famine and need to carefully manage utilization, storage and conversion of reserve products to exploitable sources of carbon and energy. Polyhydroxyalkanoates (PHAs) are storage polymers that serve bacteria as sources of food materials under physiological conditions of carbon demand. In order to obtain insights into the role of PHA depolymerase...

The aim of the present cross-sectional exploratory pilot study was to analyze ability Phase Angle (PhA) predict physical function, muscle strength and bone indicators, upon adjusting for potential confounders [age, sex, lean mass, body mass index (BMI)]. This included 56 physically independent older adults (age, 68.29 ± 3.01 years; BMI, 28.09 4.37 kg/m2). A multi-frequency segmental bioelectric...

Background: Similar to inflammatory cells, peripheral blood mononuclear cells (PBMCs) can also infiltrate in to kidney and urinary tracts and subsequently excreted by urine. In this study we determined the viability rate and response to phytohemagglutinin-A (PHA) of human PBMCs in normal urine. Methods: A number of 1×106 ficoll-hypaque isolated PBMCs were dispensed in 1 ml normal urine...

Genome-wide analysis has revealed abundant FabG (beta-ketoacyl-ACP reductase) paralogs, with uncharacterized biological functions, in several halophilic archaea. In this study, we identified for the first time that the fabG1 gene, but not the other five fabG paralogs, encodes the polyhydroxyalkanoate (PHA)-specific acetoacetyl coenzyme A (acetoacetyl-CoA) reductase in Haloarcula hispanica. Alth...

Polyhydroxyalkanoates (PHAs) are a family of biopolyesters accumulated by a variety of microorganisms as carbon and energy storage under starvation conditions. We focused on marine purple non-sulfur photosynthetic bacteria as host microorganisms for PHA production and developed a method for their isolation from natural seawater. To identify novel PHA-producing marine purple non-sulfur photosynt...

BACKGROUND Microbial polyhydroxyalkanoates (PHA) are biopolyesters consisting of diverse monomers. PHA synthase PhaC2Ps cloned from Pseudomonas stutzeri 1317 is able to polymerize short-chain-length (scl) 3-hydroxybutyrate (3HB) monomers and medium-chain-length (mcl) 3-hydroxyalkanoates (3HA) with carbon chain lengths ranging from C6 to C12. However, the scl and mcl PHA production in Escherichi...

Recently, the safety issue of maritime autonomous surface ships (MASS) has become a hot topic. Preliminary hazard analysis MASS can assist ship design and ensure safe reliable operation. However, since technology is still at its early stage, there are not enough data for comprehensive analysis. Hence, this paper attempts to combine conventional experiments conduct preliminary autonomy level III...

Muscle strength can define the general muscular fitness (MF) measurable through hand-grip strength (HG), which is a factor that relates to the health of people of different ages. In this study we evaluated the muscle strength together with a bioimpedance electric analysis in 223 healthy Colombian adult subjects. The bioelectrical impedance vector analysis (BIVA) was conducted to determine the r...