Peptide nucleic acid (PNA) has outstanding affinity over DNA for complementary nucleic acid sequences by forming a PNA-DNA heterodimer upon hybridization via Watson-Crick base-pairing. To verify whether PNA probes on an electrode surface enhance sensitivity for potentiometric DNA detection or not, we conducted a comparative study on the hybridization of PNA and DNA probes on the surface of a 10...

Experimental evidence is presented that mitochondrial oxidative phosphorylation is dependent on oxygen concentration in its physiologic range both in vivo and in vitro. Mitochondrial oxidative phosphorylation is considered to act as a tissue oxygen sensor important for controlling local vascular resistance.

Presently there are a number of fine and sensitive methods for determining chemical or biochemical substance among which bio electrodes represent the most recent development. Bioelectrodes consist of biologically active materials (e.g enzyme, antibody, whole cell or cell fragments) held in close proximity to a suitable electrochemical transducer that sense specific electroactive enzyme subs...

A fruitful combination of potentiometry, absorption spectrophotometry, ESMS and 1H NMR enabled the characterisation of two caesium complexes with norbadione A and the determination of the respective stability constants of a mononuclear and a dinuclear caesium complex at pH approximately 6; a preliminary study allowed the assignment of five protonation sites of this pigment; a positively coopera...

Photosynthetic Electron Transport, Plastidic Cytochromes, Dual-Wavelength Spectroscopy, Cross-Over Experiments; Oxidation-Reduction Potentiometry Dual-wavelength spectroscopy allows the precise and fast measurement of small absorbance changes in highly absorbing and scattering samples (fS 10“ 3 absorbance). The sensitivity of this method makes it possible to monitor directly redox changes of el...

A simple simulation using SIMULINK is presented to model the thermal/control behaviour of a domestic gas boiler. The simulation was the first step in a research programme to investigate the efficacy of control using an oxygen sensor. A brief review of boiler control is used to outline current practice and research developments. Ratio control in several forms has been investigated. Performance i...

Cytochrome P450 reductase (CPR) is a diflavin enzyme responsible for electron donation to mammalian cytochrome P450 enzymes in the endoplasmic reticulum. Dissection of the enzyme into functional domains and studies by site-directed mutagenesis have enabled detailed characterization of the mechanism of electron transfer using stopped-flow and equilibrium-perturbation methods, and redox potentiom...